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Author Hankin, Joseph A. ♦ Farias, Santiago E. ♦ Barkley, Robert M. ♦ Heidenreich, Kim ♦ Frey, Lauren C. ♦ Hamazaki, Kei ♦ Kim, Hee Yong ♦ Murphy, Robert C.
Source SpringerLink
Content type Text
Publisher Springer-Verlag
File Format PDF
Copyright Year ©2011
Language English
Subject Domain (in DDC) Natural sciences & mathematics ♦ Chemistry & allied sciences
Subject Keyword MALDI ♦ IMS ♦ Imaging mass spectrometry ♦ Sublimation ♦ Brain ♦ Phosphatidylcholine ♦ Ceramide ♦ Traumatic brain injury ♦ Ischemia reperfusion ♦ Brain injury ♦ Alkali metal adduct images ♦ CA1 ♦ Hippocampus damage ♦ Bioinformatics ♦ Organic Chemistry ♦ Biotechnology ♦ Analytical Chemistry ♦ Proteomics
Abstract Matrix-assisted laser desorption ionization/ionization imaging mass spectrometry (MALDI IMS) with a time-of-flight analyzer was used to characterize the distribution of lipid molecular species in the brain of rats in two injury models. Ischemia/reperfusion injury of the rat brain after bilateral occlusion of the carotid artery altered appearance of the phospholipids present in the hippocampal region, specifically the CA1 region. These brain regions also had a large increase in the ion abundance at m/z 548.5 and collisional activation supported identification of this ion as arising from ceramide (d18:1/18:0), a lipid known to be associated with cellular apoptosis. Traumatic brain injury model in the rat was examined by MALDI IMS and the area of damage also showed an increase in ceramide (d18:1/18:0) and a remarkable loss of signal for the potassium adduct of the most abundant phosphocholine molecular species 16:0/18:1 (PC) with a corresponding increase in the sodium adduct ion. This change in PC alkali attachment ion was suggested to be a result of edema and influx of extracellular fluid likely through a loss of Na/K-ATPase caused by the injury. These studies reveal the value of MALDI IMS to examine tissues for changes in lipid biochemistry and will provide data needed to eventually understand the biochemical mechanisms relevant to tissue injury.
ISSN 10440305
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2011-04-09
Publisher Institution The American Society for Mass Spectrometry
Publisher Place New York
e-ISSN 18791123
Journal Journal of The American Society for Mass Spectrometry
Volume Number 22
Issue Number 6
Page Count 8
Starting Page 1014
Ending Page 1021


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Source: SpringerLink