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Author Ibrahim, Muhammad ♦ Yasmeen, Sumaira ♦ Zaman, Ghanva ♦ Bin, Li ♦ Al Qurainy, Fahad ♦ Athar, Habib ur Rehman ♦ Shah, Kausar Hussain ♦ Khurshid, Muhammad ♦ Ashraf, Muhammad
Source SpringerLink
Content type Text
Publisher Springer Japan
File Format PDF
Copyright Year ©2016
Language English
Subject Domain (in DDC) Natural sciences & mathematics ♦ Life sciences; biology
Subject Keyword Cotton plant ♦ Leaf tissues ♦ LC–MS/MS ♦ COB-like proteins ♦ Cell morphogenesis ♦ Entomology ♦ Agriculture ♦ Applied Ecology ♦ Plant Pathology ♦ Zoology ♦ Environmental Management
Abstract The whitefly Bemisia tabaci Gennadius (Homoptera: Aleyrodidae) is an important pest of cotton in many countries including Pakistan. Even though it has gained resistance for various registered insecticides such as the neonicotinoid insecticide thiamethoxam, the cellular changes occurring in plants during whitefly infestation and resistance are poorly understood. In particular, the proteomic analysis of interaction between cotton plant and devastating whiteflies is poorly understood. To reveal the physiological response as well as the molecular interaction during the incursion of B. tabaci, one-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (1-D SDS-PAGE) following liquid chromatography coupled with tandem mass spectrometry (LC–MS/MS) was conducted, combined with in silico protein characterizations and protein–protein interaction following validation of LC–MS/MS results by quantitative real-time PCR. The shotgun proteomic analysis of cotton leaves infested with whitefly resulted in the identification of 38 proteins, and 24 under control (non-infested) conditions. Of them, 11 proteins were identified as shared proteins, 13 were differentially induced in non-infested conditions, and 27 were induced only under whitefly-infested conditions. The major proteins like MAP kinases, COBRA-like protein family, and NBS disease resistance protein were expressed under infested conditions; while the heat shock protein 90 and cyclic nucleotide-gated ion channel 5-like major proteins were expressed under whitefly non-infested conditions. The current manuscript presents new insights into the mechanism of whitefly infestation and our understanding of the roles of COB and COBLs in host-pest interaction. To the best of our knowledge, this is the first ever report to reveal the role of several proteins, including COB and COBLs in host-pest leaf interaction.
ISSN 00036862
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2016-08-01
Publisher Place Tokyo
e-ISSN 1347605X
Journal Applied Entomology and Zoology
Volume Number 51
Issue Number 4
Page Count 9
Starting Page 599
Ending Page 607

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Source: SpringerLink