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Author Mittal, Dinesh ♦ Jindal, Naresh ♦ Tiwari, Ashok Kumar ♦ Khokhar, Raj Singh
Source SpringerLink
Content type Text
Publisher Springer India
File Format PDF
Copyright Year ©2013
Language English
Subject Domain (in DDC) Natural sciences & mathematics ♦ Life sciences; biology
Subject Keyword Molecular characterization ♦ Broiler chickens ♦ Fowl adenovirus ♦ Hydropericardium syndrome ♦ Inclusion body hepatitis ♦ Microbiology ♦ Life Sciences ♦ Biochemistry ♦ Protein Structure ♦ Cell Biology
Abstract The present study was carried out for detection and molecular characterization of fowl adenoviruses (FAdVs) associated with hydropericardium syndrome or inclusion body hepatitis in commercial broiler chickens. The FAdVs were detected in liver samples from 33 commercial broiler chicken flocks by polymerase chain reaction (PCR) using hexon gene specific primers. The restriction enzyme analysis using StyI, BsiWI, MluI, AspI, BglI and ScaI enzymes of all the 33 FAdV-positive samples revealed FAdV-4 in 10 samples, FAdV-8 in five samples, FAdV-2 and FAdV-12 in two samples each, and FAdV-5 and FAdV-6 in one sample each. Twelve samples revealed the digestion pattern for more than one serotypes with FAdV-8 and FAdV-5, FAdV-8 and FAdV-7, FAdV-8 and FAdV-6, FAdV-8 and FAdV-12 being the predominant combinations indicating mixed infection. The serotypes FAdV-2 and FAdV-5 have not been detected previously in the country. The purified PCR products of FAdVs of four samples (HR 1, HR 2, HR 3 and HR 4) were cloned and sequenced. Phylogenetic analysis revealed that FAdVs of all four samples clustered in separate groups consistent with the REA pattern. In conclusion, this study reveals the presence of FAdV-2, FAdV-4, FAdV-5, FAdV-6, FAdV-7, FAdV-8 and FAdV-12 in broiler chickens affected with hydropericardium syndrome or inclusion body hepatitis in India.
ISSN 23473584
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2013-11-30
Publisher Place New Delhi
e-ISSN 23473517
Journal VirusDisease
Volume Number 25
Issue Number 1
Page Count 6
Starting Page 114
Ending Page 119

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Source: SpringerLink