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Author Dhar, Priyanka ♦ Kaur, Gurvinder
Source SpringerLink
Content type Text
Publisher Springer-Verlag
File Format PDF
Copyright Year ©2009
Language English
Subject Domain (in DDC) Natural sciences & mathematics ♦ Life sciences; biology
Subject Keyword Metarhizium anisopliae ♦ osmotic stabilizer ♦ protoplast ♦ mycelium age ♦ incubation ♦ Microbiology ♦ Microbial Genetics and Genomics ♦ Microbial Ecology ♦ Fungus Genetics ♦ Medical Microbiology ♦ Applied Microbiology
Abstract Optimization of different factors for efficient protoplast release fromMetarhizium anisopliae was investigated. Factors like culture media, age of the mycelium, incubation time, different enzymatic combinations and osmotic stabilizer were studied. Mycelium harvested at 40$^{th}$ h in Sabouraud Dextrose broth showed the best protoplast yield over the other media and age of mycelium tested. An incubation time of 3 h and Lysing enzyme at a concentration of 10 mg/ml was the best among the different enzymatic concentrations and combinations tested and yielded a protoplast release of 7.3×10$^{8}$ protoplasts/ml. The most suitable osmotic stabilizer for efficient protoplast release was 0.7M KCl.
ISSN 15904261
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2009-01-01
Publisher Place Berlin, Heidelberg
e-ISSN 18692044
Journal Annals of Microbiology
Volume Number 59
Issue Number 1
Page Count 4
Starting Page 183
Ending Page 186

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Source: SpringerLink