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Author Qiu, Jun Jun ♦ Guo, Jun Jie ♦ Lv, Tian Jiao ♦ Jin, Hong Yan ♦ Ding, Jing Xin ♦ Feng, Wei Wei ♦ Zhang, Ying ♦ Hua, Ke Qin
Source SpringerLink
Content type Text
Publisher Springer Netherlands
File Format PDF
Copyright Year ©2013
Language English
Subject Domain (in DDC) Technology ♦ Medicine & health
Subject Keyword CENP-A ♦ EOC ♦ Biomarker ♦ Prognosis ♦ Cancer Research
Abstract Altered expression of centromere protein-A (CENP-A) is observed in various types of human cancers. However, the clinical significance and pathological role of CENP-A in epithelial ovarian cancer (EOC) remains unclear. The main objective of this investigation was to clarify the relationships between CENP-A expression and the clinicopathological features of patients with EOC. Real-time quantitative PCR and Western blot were performed to examine CENP-A expression in 20 pairs of fresh-frozen EOC tissues and corresponding noncancerous tissues. Using immunohistochemistry, we performed a retrospective study of the CENP-A expression levels on 120 archival EOC paraffin-embedded samples. Prognostic outcomes correlated with CENP-A were examined using Kaplan–Meier analysis and Cox proportional hazards model. Our results showed that the expression levels of CENP-A mRNA and protein in EOC tissues were both significantly higher than those in noncancerous tissues. By immunohistochemistry, the data revealed that high CENP-A expression was significantly correlated with pathological grade (P = 0.02) and International Federation of Gynecology and Obstetrics stage (P = 0.006). Consistent with these results, we found that high expression of CENP-A was significantly correlated with poor survival in EOC patients (P < 0.001). Furthermore, Cox regression analyses showed that CENP-A expression was an independent predictor of overall survival. Our data suggest that CENP-A could play an important role in EOC and might serve as a valuable prognostic marker and potential target for gene therapy in the treatment of EOC.
ISSN 10104283
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2013-05-28
Publisher Place Dordrecht
e-ISSN 14230380
Journal Tumor Biology
Volume Number 34
Issue Number 5
Page Count 5
Starting Page 2971
Ending Page 2975

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Source: SpringerLink