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Author Kim, Woong Ki ♦ Corey, Sarah ♦ Chesney, Gillian ♦ Knight, Heather ♦ Klumpp, Sherry ♦ Wüthrich, Christian ♦ Letvin, Norman L. ♦ Koralnik, Igor J. ♦ Lackner, Andrew ♦ Veasey, Ronald S. ♦ Williams, Kenneth
Source SpringerLink
Content type Text
Publisher Springer-Verlag
File Format PDF
Copyright Year ©2004
Language English
Subject Domain (in DDC) Technology ♦ Medicine & health
Subject Keyword brain ♦ perivascular macrophages ♦ SIV encephalitis ♦ T lymphocytes ♦ Neurosciences ♦ Virology ♦ Infectious Diseases ♦ Immunology ♦ Neurology
Abstract T lymphocytes are found within brains infected with human immunodeficiency virus (HIV) or simian immunodeficiency virus (SIV) where they are a minor, but consistently identified, population. However, little analysis of their phenotypes has been done, and questions concerning whether or not they are viral antigen specific has not been thoroughly examined. We investigated the central nervous system (CNS) of SIV-infected rhesus macaques to identify T-lymphocyte subsets in relation to virus-infected cells and brain microvessels. We have found that a sensitive antigen-retrieval technique greatly enhanced immunohistochemical detection of CD4$^{+}$ and CD8$^{+}$ T lymphocytes in control studies. In encephalitic brains of SIV-infected monkeys with acquired immunodeficiency syndrome (AIDS), we found a significant accumulation of CD8$^{+}$ T lymphocytes but little-to-no accumulation of CD4$^{+}$ T lymphocytes. CD4$^{+}$ cells, when detected, were mostly monocyte/macrophages closely associated with CNS vessels. Using a combination of in situ hybridization for SIV RNA, and immunohistochemistry for CD8$^{+}$ T lymphocytes and/or Glut-1 for endothelial cells on brain microvessels, we found CD8$^{+}$ T lymphocytes with an angiocentric distribution often adjacent to virus-infected cells. In the CNS of animals with SIV encephalitis, there was a trend of CD8$^{+}$ T lymphocytes that were not directly juxtaposed with CNS vessels. These data suggest that in brains of SIV-infected monkeys and HIV-infected humans, CD8$^{+}$ T lymphocytes traffic to and are retained in the CNS in an angiocentric and possibly antigen-specific manner.
ISSN 13550284
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2004-01-01
Publisher Place New York
e-ISSN 15382443
Journal Journal of NeuroVirology
Volume Number 10
Issue Number 5
Page Count 11
Starting Page 315
Ending Page 325

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Source: SpringerLink