|Author||Jeembaeva, Meerim ♦ Jönsson, B. ♦ Castelnovo, Martin ♦ Evilevitch, Alex|
|Date of Submission||2010-01-06|
|Subject Domain (in DDC)||Computer science, information & general works ♦ Natural sciences & mathematics ♦ Physics ♦ Life sciences; biology|
|Subject Keyword||Physics - Biological Physics ♦ Quantitative Biology - Subcellular Processes ♦ physics:physics ♦ q-bio|
|Abstract||Most bacteriophages are known to inject their double-stranded DNA into bacteria upon receptor binding in an essentially spontaneous way. This downhill thermodynamic process from the intact virion toward the empty viral capsid plus released DNA is made possible by the energy stored during active packaging of the genome into the capsid. Only indirect measurements of this energy have been available until now using either single-molecule or osmotic suppression techniques. In this paper, we describe for the first time the use of isothermal titration calorimetry to directly measure the heat released (or equivalently the enthalpy) during DNA ejection from phage lambda, triggered in solution by a solubilized receptor. Quantitative analyses of the results lead to the identification of thermodynamic determinants associated with DNA ejection. The values obtained were found to be consistent with those previously predicted by analytical models and numerical simulations. Moreover, the results confirm the role of DNA hydration in the energetics of genome confinement in viral capsids.|
|Learning Resource Type||Article|
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