### SURVIVAL KINETICS OF HeLa S-3 CELLS AFTER INCORPORATION OF $sup 3$H- THYMIDINE OR $sup 3$H-URIDINESURVIVAL KINETICS OF HeLa S-3 CELLS AFTER INCORPORATION OF $sup 3$H- THYMIDINE OR $sup 3$H-URIDINE

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 Author Marin, G. ♦ Bender, M. A. Source United States Department of Energy Office of Scientific and Technical Information Content type Text Language English
 Subject Keyword BIOLOGY AND MEDICINE ♦ ANIMAL CELLS ♦ BETA DECAY ♦ CELL NUCLEI ♦ COMPUTERS ♦ DIGITAL SYSTEMS ♦ DNA ♦ HELA CELLS ♦ HETEROCYCLICS ♦ LABELLED COMPOUNDS ♦ METABOLISM ♦ MITOSIS ♦ NUCLEIC ACIDS ♦ NUCLEOSIDES ♦ ORGANIC NITROGEN COMPOUNDS ♦ RIBONUCLEIC ACID ♦ SURVIVAL TIME ♦ THYMIDINE ♦ TISSUES ♦ TRACER TECHNIQUES ♦ TRITIUM ♦ URIDINE ♦ VARIATIONS ♦ X RADIATION Abstract The survival kinetics of mammalian tissue culture cells containing tritium-labeled nucleosides were studied. He La S-3 cells were allowed to incorporate various amounts of /sup 3/H-thymidine or /sup 3/H-uridine into their DNA or RNA. Owing to the short mean free path of the tritium-decay BETA -ray, incorporation of /sup 3/H-thymidine irratliates mainly the nucleus, while most of the radiation from the /sup 3/H-uridine is absorbed by the cytoplasm. A model which takes into account the persistence of BETA radiation from the labeled nucleic acids and the dose rate reduction due to cell division was constructed for the expected survival kinetics. The experimental data were fitted to this model by means of a digital computer. It was found that the hit number was not significantly different from the values previously reported for x rays. It was concluded that all the cell killing seen can be accounted for by the BETA radiation to which the cell is exposed. (auth) Educational Use Research Learning Resource Type Article Publisher Date 1963-09-01 Journal Intern. J. Radiation Biol. Volume Number 7 Organization Oak Ridge National Lab., Tenn.