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Author Greenberg, S. A. ♦ Do, K. ♦ Grey, P. A.
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT. ♦ BASIC BIOLOGICAL SCIENCES ♦ BARBITURATES ♦ TOXICITY ♦ BRAIN ♦ METABOLISM ♦ ETHANOL ♦ PROTEINS ♦ BIOSYNTHESIS ♦ CARBON 14 COMPOUNDS ♦ CYCLOHEXIMIDE ♦ LEUCINE ♦ RATS ♦ TRACER TECHNIQUES ♦ ALCOHOLS ♦ AMINO ACIDS ♦ ANESTHETICS ♦ ANIMALS ♦ ANTI-INFECTIVE AGENTS ♦ ANTIBIOTICS ♦ AZINES ♦ BODY ♦ CARBOXYLIC ACIDS ♦ CENTRAL NERVOUS SYSTEM ♦ CENTRAL NERVOUS SYSTEM DEPRESSANTS ♦ DRUGS ♦ FUNGICIDES ♦ HETEROCYCLIC COMPOUNDS ♦ HYDROXY COMPOUNDS ♦ HYPNOTICS AND SEDATIVES ♦ ISOTOPE APPLICATIONS ♦ LABELLED COMPOUNDS ♦ MAMMALS ♦ NERVOUS SYSTEM ♦ ORGANIC ACIDS ♦ ORGANIC COMPOUNDS ♦ ORGANIC NITROGEN COMPOUNDS ♦ ORGANIC OXYGEN COMPOUNDS ♦ ORGANS ♦ PESTICIDES ♦ PYRIMIDINES ♦ RODENTS ♦ SYNTHESIS ♦ VERTEBRATES 560300* -- Chemicals Metabolism & Toxicology ♦ BiochemistryTracer Techniques
Abstract Central nervous system (CNS) depressants such as ethanol and barbiturates under acute or chronic conditions can induce changes in rat brain protein synthesis. While these data demonstrate the individual effects of drugs on protein synthesis, the response of brain protein synthesis to alcohol-drug interactions is not known. The goal of the present study was to determine the individual and combined effects of ethanol and sodium barbital on brain protein synthesis and gain an understanding of the mechanisms by which these alterations in protein synthesis are produced. Specifically, the in vivo and in vitro effects of sodium barbital (one class of barbiturates which is not metabolized by the hepatic tissue) were examined on brain protein synthesis in rats made physically dependent upon ethanol. Using cell free brain polysomal systems isolated from Control, Ethanol and 24 h Ethanol Withdrawn rats, data show that sodium barbital, when intubated intragastrically, inhibited the time dependent incorporation of /sup 14/C) leucine into protein by all three groups of ribosomes. Under these conditions, the Ethanol Withdrawn group displayed the largest inhibition of the /sup 14/C) leucine incorporation into protein when compared to the Control and Ethanol groups. In addition, sodium barbital when added at various concentrations in vitro to the incubation medium inhibited the incorporation of /sup 14/C) leucine into protein by Control and Ethanol polysomes. The inhibitory effects were also obtained following preincubation of ribosomes in the presence of barbital but not cycloheximide. Data suggest that brain protein synthesis, specifically brain polysomes, through interaction with ethanol or barbital are involved in the functional development of tolerance. These interactions may occur through proteins or polypeptide chains or alterations in messenger RNA components associated with the ribosomal units.
Educational Use Research
Learning Resource Type Article
Publisher Date 1987-01-01
Publisher Place United States
Journal Alcohol Drug Res.
Volume Number 4
Organization Univ. of California, Irvine


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