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Author Pollock, Tyler ♦ Tang, Brandon ♦ Catanzaro, Denys de
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword APPLIED LIFE SCIENCES ♦ ANIMAL TISSUES ♦ ANTIMICROBIAL AGENTS ♦ BLOOD SERUM ♦ BRAIN ♦ CARBON 14 ♦ ENZYMES ♦ EPOXIDES ♦ HEART ♦ IN VIVO ♦ LIQUIDS ♦ LUNGS ♦ METABOLISM ♦ MICE ♦ MUSCLES ♦ OVARIES ♦ PLASTICS ♦ POLYCARBONATES ♦ RADIOACTIVITY ♦ SCINTILLATION COUNTING ♦ UTERUS
Abstract Current human generations are commonly exposed to both triclosan (TCS), an antimicrobial agent, and bisphenol A (BPA), the monomer of polycarbonate plastics and epoxies. Both are readily absorbed into circulation and found distributed among diverse tissues. Potential interactions between TCS and BPA are largely unstudied. We investigated whether TCS exposure affects the distribution of ingested {sup 14}C-BPA in select tissues. CF-1 mice were each subcutaneously injected with TCS then orally administered 50 μg/kg {sup 14}C-BPA. Females received 0, 0.2, 0.6, 1, 2, or 18 mg TCS (equivalent respectively to 0, 6.3, 16.9, 30.1, 60.5, and 558.9 mg/kg). Males received 0, 0.2, 2, or 18 mg TCS (equivalent respectively to 0, 5.3, 53.4, and 415.0 mg/kg). Levels of radioactivity were measured through liquid scintillation counting in blood serum and brain, reproductive, and other tissues. Significantly elevated levels of radioactivity were observed following combined TCS and {sup 14}C-BPA administration, with minimally effective TCS doses being tissue-dependent (Females: lungs, 0.6 mg; uterus, 1 mg; heart, muscle, ovaries, and serum, 18 mg. Males: serum, 0.2 mg; epididymides, 2 mg). Subsequently, we found that 2 or 6 mg TCS increased radioactivity in the ovaries and serum of females orally given only 5 μg/kg {sup 14}C-BPA. These data indicate that TCS can interact with BPA in vivo, magnifying its presence in certain tissues and serum. The data are consistent with evidence that TCS utilizes enzymes that are critical for metabolism and excretion of BPA. Further research should investigate the mechanisms through which these two chemicals interact at environmentally-relevant doses. - Highlights: • We examined whether triclosan exposure affects the distribution of oral {sup 14}C-BPA. • Radioactivity was elevated in select tissues of mice injected sc with triclosan. • In females, this effect was most pronounced in the uterus, ovaries, and lungs. • In males, this effect was most prominent in the blood serum and epididymides. • Our data accord with evidence that triclosan competes for enzymes conjugating BPA.
ISSN 0041008X
Educational Use Research
Learning Resource Type Article
Publisher Date 2014-07-15
Publisher Place United States
Journal Toxicology and Applied Pharmacology
Volume Number 278
Issue Number 2


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