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Author Dziong, D. ♦ Bagnaninchi, P.O. ♦ Kearney, R.E. ♦ Tabrizian, M.
Sponsorship IEEE Engineering in Medicine and Biology Society ♦ IEEE Systems, Man, and Cybernetics Society ♦ IEEE Nanotechnology Council ♦ IEEE Computational Intelligence Society ♦ IEEE Robotics and Automation Society ♦ IEEE Computer Society
Source IEEE Xplore Digital Library
Content type Text
Publisher Institute of Electrical and Electronics Engineers, Inc. (IEEE)
File Format PDF
Copyright Year ©2002
Language English
Subject Domain (in DDC) Natural sciences & mathematics ♦ Physics ♦ Electricity & electronics ♦ Technology ♦ Medicine & health ♦ Engineering & allied operations
Subject Keyword In vitro ♦ Monitoring ♦ Polymers ♦ Permittivity measurement ♦ Probes ♦ Admittance measurement ♦ Impedance measurement ♦ Frequency measurement ♦ Coaxial components ♦ Apertures ♦ complex permittivity measurement ♦ Cell and tissue growth monitoring ♦ microporous polymer scaffolds ♦ online ♦ nondestructive
Abstract We present a system for the online, in vitro, nondestructive monitoring of tissue growth within microporous polymer scaffolds. The system is based on measuring the admittance of the sample over a frequency range of 10-200 MHz using an open-ended coaxial probe and impedance analyzer. The sample admittance is related to the sample complex permittivity (CP) by a quasi-static model of the probe's aperture admittance. A modified effective medium approximation is then used to relate the CP to the cell volume fraction. The change of cell volume fraction is used as a measure of tissue growth inside the scaffold. The system detected relative cell concentration differences between microporous polymer scaffolds seeded with 0.4, 0.45, 0.5, and 0.6 x 10<sup>6</sup> pre-osteoblast cells. In addition, the pre-osteoblast proliferation within 56 scaffolds over 14 days was recorded by the system and a concurrent DNA assay. Both techniques produced cell proliferation curves that corresponded to those found in literature. Thus, our data confirmed that the new system can assess relative cell concentration differences in microporous scaffolds enabling online nondestructive tissue growth monitoring.
Description Author affiliation :: McGill Univ., Montreal
ISSN 15361241
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2007-09-01
Publisher Place U.S.A.
Rights Holder Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Volume Number 6
Issue Number 3
Size (in Bytes) 2.13 MB
Page Count 10
Starting Page 249
Ending Page 258


Source: IEEE Xplore Digital Library