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Author Borek, C. ♦ Cleaver, J. E.
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT. ♦ AMIDES ♦ BIOLOGICAL EFFECTS ♦ ONCOGENIC TRANSFORMATIONS ♦ BIOLOGICAL PATHWAYS ♦ ADP ♦ BIOLOGICAL RADIATION EFFECTS ♦ CARCINOGENS ♦ CLONE CELLS ♦ FIBROBLASTS ♦ INHIBITION ♦ MICE ♦ TUMOR PROMOTERS ♦ X RADIATION ♦ ANIMAL CELLS ♦ ANIMALS ♦ CELL CULTURES ♦ CELL TRANSFORMATIONS ♦ CONNECTIVE TISSUE CELLS ♦ ELECTROMAGNETIC RADIATION ♦ IONIZING RADIATIONS ♦ MAMMALS ♦ NUCLEOTIDES ♦ ORGANIC COMPOUNDS ♦ ORGANIC NITROGEN COMPOUNDS ♦ PROMOTERS ♦ RADIATION EFFECTS ♦ RADIATIONS ♦ RODENTS ♦ SOMATIC CELLS ♦ VERTEBRATES ♦ Radiation Effects on Cells- External Source- (-1987) ♦ Chemicals Metabolism & Toxicology- Cells- (-1987)
Abstract Transformation of mouse C3H 10T1/2 cells by X-irradiation in vitro was blocked by the addition of 1 mM 3-aminobenzamide, an inhibitor of polyadenosine diphosphoribose (poly(ADP-ribose)) synthesis immediately after irradiation. 3-Aminobenzamide also inhibited an increase in the frequency of transformants caused by the addition of the tumor promoter, 12-O-tetradecanoylphorbol-13-acetate, 7 days after irradiation. These results demonstrate a role for poly(ADP-ribose) synthesis during the initiation and promotion stages of transformation. From previous studies it is known that poly(ADP-ribose) synthesis is stimulated by the DNA damage caused by X rays during initiation. During promotion, however, 12-O-tetradecanoylphorbol-13-acetate acted as a mitogen but did not induce detectable DNA damage, and we could detect no stimulation of poly(ADP-ribose) synthetase. The roles of poly(ADP-ribose) during initiation and during promotion must, therefore, be significantly different.
Educational Use Research
Learning Resource Type Article
Publisher Date 1986-02-13
Publisher Place United States
Journal Biochem. Biophys. Res. Commun.
Volume Number 3
Organization Columbia Univ. College of Physicians and Surgeons, New York, NY


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