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Author Pakzad*, S.R. ♦ Rahimifard, N. ♦ Hamedani, F. Hosseini ♦ Mojab, F. ♦ Bagheri, F.
Source Directory of Open Access Journals (DOAJ)
Content type Text
Publisher Iranian Society of Pharmacognosy
File Format PDF
Date Created 2017-12-23
Copyright Year ©2017
Language English
Subject Domain (in LCC) RS1-441
Subject Keyword Medicine ♦ MTT ♦ Hammada salicornica ♦ Cytotoxicity ♦ Pharmacy and materia medica
Abstract Background and objectives: A pharmaceutical products that is planned to be used in clinic, should not only have beneficial effects but also be safe too. Preclinical studies in animals are costly and need considering ethical issues. Cell culture can be used before animal tests. Considering useful effects of these methods, we have evaluated safety of total methanol extract of Hammada salicornica and its aqueous and petroleum ether fractions in cell culture.Methods: Total methanol extract was prepared with the standard method of maceration. Different fractions were prepared by liquid-liquid fractionation and the extracts were then dried with rotary evaporator. After determination of bactericidal concentration of the extracts, 400 ug/mL, the cytotoxicity was tested at various concentrations regarding the minimum antibacterial concentration by MTT test. Hep-2c and VERO cell lines were used in MTT test. A range of concentrations (10-500 ug/mL) of the extracts were prepared and were added to about 70% confluent 96 well plates. After exposure for 48 h, MTT solution was added to the wells, and 4 h later formazan crystals were solubilized and optical densities were read at 570 nm. Results: Cytotoxicity Index was calculated and significance test was performed using t-test comparing the Index of the test and control group at each concentration. No significant difference was observed. Conclusion: Various fractions of H. salicornica were not cytotoxic at concentrations above bactericidal concentrations (up to 500 ug/mL). The results need to be confirmed in animal studies before using in human subjects.
ISSN 23454458
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study
Learning Resource Type Article
Publisher Date 2017-11-01
e-ISSN 23454458
Journal Research Journal of Pharmacognosy
Volume Number 4
Issue Number Supplement
Page Count 1
Starting Page 3
Ending Page 3


Source: Directory of Open Access Journals (DOAJ)