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Author Parkes, H. ♦ Collis, P. ♦ Baildam, A. ♦ Ralphs, D. ♦ Lyons, B. ♦ Howell, A. ♦ Craig, R.
Source PubMed Central
Content type Text
Publisher Nature Publishing Group
File Format PDF
Language English
Difficulty Level Medium
Subject Domain (in DDC) Technology ♦ Medicine & health
Abstract In order to identify potential markers of prognosis in breast cancer, representative cDNA libraries were constructed using RNA isolated from primary breast tumour tissue associated with good and poor prognosis. Cross-screening of these libraries repeatedly identified cloned mRNA species associated with the immune system, in particular B-cells, in libraries derived from tumours of poor prognosis. We have used one of these a kappa IV light chain cDNA probe, in two complementary studies to investigate the relationship between immunoglobin gene expression and prognosis. The results obtained using a combination of S1 mapping, RNA blotting and in situ hybridisation demonstrate that the presence of plasma cells, as defined by infiltrating cells which express high levels of immunoglobulin kappa-chain mRNA, is associated with a poor prognosis.
ISSN 15321827
Age Range above 22 year
Educational Use Research
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 1988-12-01
Rights Holder Nature Publishing Group
e-ISSN 15321827
Journal British Journal of Cancer
Volume Number 58
Issue Number 6
Starting Page 715


Source: PubMed Central