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Author Chabrier, P. E. ♦ Roubert, P. ♦ Braquet, P.
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword RADIOLOGY AND NUCLEAR MEDICINE ♦ BLOOD-BRAIN BARRIER ♦ PHYSIOLOGY ♦ PEPTIDES ♦ RADIORECEPTOR ASSAY ♦ BRAIN ♦ CAPILLARIES ♦ CEREBRUM ♦ IODINE 125 ♦ RECEPTORS ♦ BETA DECAY RADIOISOTOPES ♦ BLOOD VESSELS ♦ BODY ♦ CARDIOVASCULAR SYSTEM ♦ CENTRAL NERVOUS SYSTEM ♦ DAYS LIVING RADIOISOTOPES ♦ ELECTRON CAPTURE RADIOISOTOPES ♦ INTERMEDIATE MASS NUCLEI ♦ IODINE ISOTOPES ♦ ISOTOPE APPLICATIONS ♦ ISOTOPES ♦ MEMBRANE PROTEINS ♦ NERVOUS SYSTEM ♦ NUCLEI ♦ ODD-EVEN NUCLEI ♦ ORGANIC COMPOUNDS ♦ ORGANS ♦ PROTEINS ♦ RADIOISOTOPES ♦ TRACER TECHNIQUES ♦ Medicine- Unsealed Radionuclides in Diagnostics
Abstract Cerebral capillaries constitute the blood-brain barrier. Studies of specific receptors (neurotransmitters or hormones) located on this structure can be performed by means of radioligand-binding techniques on isolated brain microvessels. The authors examined on pure bovine cerebral microvessel preparations the binding of atrial natriuretic factor (ANF), using /sup 125/I-labeled ANF. Saturation and competition experiments demonstrated the presence of a single class of ANF-binding sites with high affinity and with a binding capacity of 58 fmol/mg of protein. The binding of /sup 125/I-labeled ANF to brain microvessels is specific, reversible, and time dependent, as is shown by association-dissociation experiments. The demonstration of specific ANF-binding sites on brain microvessels supposes a physiological role of ANF on brain microvasculature. The coexistence of ANF and angiotensin II receptors on this cerebrovascular tissue suggests that the two circulating peptides may act as mutual antagonists in the regulation of brain microcirculation and/or blood-brain barrier function.
Educational Use Research
Learning Resource Type Article
Publisher Date 1987-04-01
Publisher Place United States
Volume Number 84
Issue Number 7
Organization Institut Henri Beaufour Research Labs., Les Ulis, France


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