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Researcher Shinde, K. S.
Advisor Aher, V. D.
Source KrishiKosh-Indian National Agricultural Research System
Content type Text
Educational Degree Master of Science (M.Sc.)
Publisher MAFSU
File Format PDF
Language English
Subject Domain (in DDC) Technology ♦ Agriculture & related technologies ♦ Animal husbandry
Subject Keyword Clinical Evaluation of Platelet Rich Plasma On Fracture Healing In Caprine ♦ Veterinary Surgery and Radiology
Abstract Twelve clinical cases of fracture in goat of both sex aging one and half to six months were selected for study. These cases were divided in to two groups viz. group A group and group B each group having 6 animals each. Group A consists of cases of closed fracture treated with reduction and immobilization by applying plaster of paris cast and this group was kept as control group. In group B, in addition to reduction and immobilization of closed fracture, injection of 1ml autogenous PRP was given at the site on the day of immobilization, 3rd and 7th day of treatment. Fracture healing in both groups was evaluated on the basis of clinical, radiological and biochemical observations. Clinical observation was recorded on 0, 3rd, 7th and 14th day. Heart rate, rectal temperature and respiration rate were found increased on day of admission and later decreased gradually and remain constant up to 28th day within normal range as a result of rigid and compact stabilization of fracture fragments with plaster of paris cast. Weight bearing was recorded on 0, 7th, 14th, 21st and 45th day post immobilization. In present study it was observed that on day 0, all the animals of both groups were unable to bear their weight on the affected limb. On 7th day 5 animals of group A and 2 animals of group B exhibited poor grade weight bearing with moderate pain and 4 animals of group B and one animal of group A showed (fair grade) slight to moderate lameness with consistent weight bearing. On 14th day good grade of weight bearing was observed in 3 animals of group B whereas majority of animals of group A showed fair grade of weight bearing. At 21st day, in 4 cases of group A and all 6 cases of group B good grade of weight bearing with slight lameness was evident. At 45th day, in all 6 cases of group B exhibited excellent grade of weight bearing without lameness. 3 animals in group A showed excellent grade of weight bearing without lameness while remaining 3 showed good grade of weight bearing with slight lameness. Weight bearing on the fracture limb was much early and excellent in all goats of group B as compared to animals of group A. Radiological evaluation of each fracture case was done on day 0 before and after immobilization and on 15th, 30th and 45th day. On day 0 radiographic examination revealed uneven edges, cracking, angulation, overriding of bone fragment in both groups. On 15th day in group A revealed slight periosteal reaction with formation of mild callus in 4 cases. In group B animals higher degree of periosteal reaction with lesser amount of fracture gap than that of group A animals. On day 30th the periosteal and endosteal reaction with callus formation was good grade in 3 animals and remaining 3 animals showed fair grade periosteal reaction in animals of group A and radiograph of group B depicted (Good grade) bridging of cortex along and calcification in 5 cases and periosteal reaction with soft callus in 1 case. On 45th day Fracture line was almost invisible with bridging in group B whereas in group A fracture line was still visible which indicative of healing of fracture comparatively slower than group B. Biochemical evaluation of fracture was done on 0, 3rd, 7th, 14th and 28th day which revealed no any significant changes except the serum alkaline phosphatase which was significantly high on 14th day in group B and later on decreased up to 28th day. Estimation of serum during fracture healing revealed non- significant steady inclination of serum calcium in both groups up to 28th day. The serum phosphorous values showed no any significant difference with fluctuation within normal range. Autologous PRP was prepared using a simple table top centrifuge system which found to be easy and feasible under field condition. The mean ± S.E. platelet concentration in PRP before addition of activated thrombin rich plasma (TRP) was 352±12.27 x 103 platelets/μl. From the present clinical study it could be concluded that Autogenous Platelet rich plasma (PRP) injection into fracture site could enhance bone healing and can effectively decrease treatment time.
Educational Use Research
Education Level UG and PG
Learning Resource Type Thesis
Publisher Place Nagpur
Size (in Bytes) 5.04 MB
Page Count 95