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Researcher Ray, Susim Mukul
Advisor Singh, Ajit
Source KrishiKosh-Indian National Agricultural Research System
Content type Text
Educational Degree Master of Science (M.Sc.)
Publisher LUVAS
File Format PDF
Language English
Subject Domain (in DDC) Technology ♦ Agriculture & related technologies ♦ Animal husbandry
Subject Keyword Aluminium Nanoparticles ♦ Klh ♦ Adjuvants ♦ Diva ♦ Bacterin ♦ Veterinary Microbiology ♦ Studies On Novel Haemorrhagic Septicaemia (h.s.) Vaccine Formulations Incorporating Aluminium Oxide Nanoparticles and Keyhole Limpet Hemocyanin
Abstract Haemorrhagic septicaemia (HS) caused by Pasteurella multocida B:2 serotype is a major cause of morbidity and mortality in cattle and buffaloes in India and other Asian countries. Alum adjuvanted and oil emulsion commercial vaccines for HS control suffer from potency, efficacy, safety- related problems, and are unable to differentiate infected from vaccinated animals. The present study aimed to improve HS vaccine qualities by using aluminium nanoparticles and keyhole limpet hemocyanin (KLH) as adjuvants, and to develop a DIVA strategy by incorporating KLH. Aluminium nanoparticles and KLH were mixed with formalin-inactivated Pasteurella multocida B:2 P52 strain. Various controls were also used. Swiss albino mice (n=8/group) were inoculated twice at three-week interval. Two weeks after booster, mice were challenged with 50xlethal dose50 of virulent homologous bacteria (500 CFUs/mouse). All the mice (n=8) immunized with nanoparticles + KLH + bacterin (HSVac-I) survived the challenge. While aluminium nanoparticles (HSVac-II), KLH (HSVac-III), plain-bacterin (HSVac-IV) and alum-precipitated bacterin (HSVac-V) groups (n=8/group) had seven, five, five and one survivors, respectively. Anti-bacterial antibody levels in the HSVac-I,-II &-III groups were significantly higher than those of HSVac-V (p<0.001) and HSVac-IV (p<0.05) groups from ‘d14’ post-immunization. Antisera from different groups (HSVac-I,-II &-III) of mice reacted strongly with LPS and 32-37 kDa polypeptide bands in BEA immunoblots. Several polypeptide bands were observed in KLH profiles in polyacrylamide gel electrophoresis. Anti-KLH antibodies were produced in high titres in all the KLH groups and persisted as long as those against bacterial antigens had. HSVac-I group antisera also reacted strongly with some polypeptides of KLH immunoblots. Anti-KLH antibodies were absent in sera samples from cattle, buffaloes, sheep and goats. In conclusion, efficacy and potency of HS bacterin was improved by aluminium nanoparticles and KLH. KLH inclusion in the experimental vaccine was also suitable as a marker for DIVA strategy for HS control programme.
Educational Use Research
Education Level UG and PG
Learning Resource Type Thesis
Size (in Bytes) 2.28 MB