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Researcher KAPALE VIJAY PANDURANG
Advisor Singh, Archana
Source KrishiKosh-Indian National Agricultural Research System
Content type Text
Educational Degree Master of Science (M.Sc.)
Publisher DIVISION OF BIOCHEMISTRY ICAR-INDIAN AGRICULTURAL RESEARCH INSTITUTE NEW DELHI
File Format PDF
Language English
Subject Domain (in DDC) Natural sciences & mathematics ♦ Life sciences; biology ♦ Biochemistry
Subject Keyword Biochemical and Molecular Characterization of Salt Tolerance In Contrasting Pigeon Pea Genotypes ♦ Biochemistry
Abstract igeon pea[Cajanuscajan (L.) Millsp], a short-lived perennial shrub, commonly known as ‘Arhar’ or ‘Tuhr’ is one of the major grain legume (pulse) crops of the Indian subcontinent. It has a diploid genome with 11 pairs of chromosomes and a genome size estimated to be 858 Mbp. Salinity being a critical factor to sustainable pigeon pea production addresses limitations to meet the demands of resource-poor people where it is grown. Based onpercentgermination, vigor and salt tolerance, out of total 175 pigeon pea genotypes,12 genotypes were selected for morphological screening. Prior to this, different concentrations (0-300mMNaCl) and durations (0,7, 14, 21 days) of salt stress imposition werealso determined.Salt stress with 250mMNaCl for 7 days of stress treatment was found to be optimum for further biochemical and molecular studies. Six most salt responsive pigeon pea genotypes, three from each group of salt-tolerant (ICP6815, ICP7, ICP8860) and salt-susceptible (ICP1071, ICP14155, ICP15185) genotypes were subjected for morphological and biochemical screening toidentify one each of most salt-tolerant and salt-susceptible contrasting pigeon pea genotype. Biochemical studies-antioxidant potential (AO), lipid peroxidation (LP)and total phenolic contents (TPC)were carried out in root and shoot tissues of screened 6 genotypes.Based on morphological and biochemical expressions, ICP7 and ICP1071 were found to be most salt-tolerant and salt-susceptible pigeon pea genotypes, respectively. Biochemical expressions were found to more prominent in the shoot as compared to the roots with reference to phenol content and lipid peroxidation but in the case of antioxidant potential it was more or less same. Findings showed that TPC, LP and AO activities are determinant biochemical markers for saltstress tolerance. A progressive increase in TPC and AO activity were observed during saltstress imposition in the salt-tolerant genotype than salt-susceptible and LP was found to be in reverse pattern. Partial CDS for pigeon pea stress-responsive genes viz., hybrid prolinerich protein (CcHyPRP,218 bp), cold, drought salt regulatory protein (CcCDR,207 bp) and cyclophilin protein (CcCYP,323 bp) conferring abiotic tolerance were cloned from ICP7 ii genotype and sequences were submitted to NCBI with the Accession Nos. KP996197, KP996198 and KR262820, respectively.The BLAST search sequence homology characterization revealed their 86-99% similarity with CajanuscajanandGlycine max.The semi-quantitative (RT-PCR) expression analysis of these isolated genes were conducted in root and shoot tissues of contrasting pigeon pea genotype and found to be expressed in both root and shoot tissues.These findings shall go a long way not only in understanding the important molecular and biochemical mechanisms underlying the salinity tolerance of the pigeon but also can be utilized in providing important leads to the breeders to evolve improved varieties of pigeon pea with higher salinity tolerance levels.
Educational Use Research
Education Level UG and PG
Learning Resource Type Thesis
Size (in Bytes) 6.92 MB