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Researcher Kumar, Jitender
Advisor Jain, R. K.
Source KrishiKosh-Indian National Agricultural Research System
Content type Text
Educational Degree Doctor of Philosophy (Ph.D.)
Publisher CCSHAU
File Format PDF
Language English
Subject Domain (in DDC) Technology ♦ Agriculture & related technologies ♦ Animal husbandry
Subject Keyword Biotechnology and Molicular Biology ♦ Oryza Sativa ♦ Micronutrient ♦ Microsatellite (ssr) Markers ♦ Qtls ♦ Lod
Abstract Molecular markers can greatly improve the efficacy of rice breeding programs to improve the mineral (iron and zinc) content and bioavailability. Two F2 populations (PAU201 x Palman 579 and HKR47 x BR 4-10) and 8 BC 1 plants (PAU201 x Palman 579/PAU201) derived from crosses between iron rich (Palman 579 and BR 4-10) and high yielding indica rice varieties (PAU201 and HKR47) were evaluated for various agronomic traits and mineral (Fe and Zn) content. Both the segregating F 2 populations displayed large variation for plant height (55.8-102.3 and 77.5-110.2 cm), effective number of tillers per plant (2-20 and 3-20), panicle length (15.9-25.4 and 16.3-32.03), grain yield per plant (1.93-41.53 and 3.32-59.06 g), 1000 grain weight (11.57-29.15 and 16.31-34.43 g), grain length/breadth ratio (2.90-5.10 and 2.70-4.41), Fe content (18.6 to 475.4 µg/g and 7.3 to 146.5 µg/g) and Zn content (4.4 to 157.4 µg/g and 12.5 to 92.9 µg/g), respectively. Variation for Fe (69.4 to 86.1 µg/g) and Zn (21.5 to 95.6 µg/g) contents was also observed in BC 1 plants. One of the PAU201 x Palman 579 F 2 plants, had exceptionally higher iron (475.4 µg/g) and zinc (157.4 µg/g) contents in comparison to the donor parent, Palman 579 (Fe-382.36 µg/g, Zn-21.6 µg/g). Transgressive segregation for grain iron and/or zinc contents was noticed in F 2 individuals of both the crosses. Grain iron content showed significant positive correlation (r=0.523 and 0.299) with grain zinc content in both the F 2 populations indicating the feasibility of improving Fe and Zn levels simultaneously in rice grain. As many as 76/100 SSRs, which showed polymorphism among the two parental rice genotypes, were used to map the QTLs associated with mineral content in grains using 50 PAU201 x Palman 579 F 2 plants displaying the entire range of variation in Fe and Zn contents. A total of 171 alleles were identified in 50 PAU201 x Palman 579 F 2 plants and 14 new recombinant alleles (different that those in parent rice varieties) were identified. NTSYS-pc based two dimensional PCA analysis showed scattering of the F 2 population between the two distinct parental genotypes. Composite interval mapping (CIM) analysis by WinQTL cartographer 2.5 revealed a total of eleven QTLs for mineral content in grains on chromosome 2, 3, 7, 10 and 12. Eight QTLs for Fe content were mapped on chromosome 2 (qFE1, qFE2 and qFE3 at map positions 54.4, 138.6, 159.2 cM espectively), chromosome 3 (qFE4 at map position 54.6cM), chromosome 7 (qFE5 at map position 31.3 cM), chromosome 10 (qFE6 and qFE7 at map positions 25.9, 108.0 cM respectively) and chromosome 12 (qFE8 at map position 60.3 cM). In addition, three QTLs (qZN1, qZN2 and qZN3) for Zn content were identified on chromosome 2 (qZN1) and 10 (qZN2 and qZN3) with the map positions of 65.5, 23.8 and 115.9 cM respectively.
Educational Use Research
Education Level UG and PG
Learning Resource Type Thesis
Size (in Bytes) 2.94 MB