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Researcher Jagdale, Vijay Rajaram
Advisor Punia, M. S.
Source KrishiKosh-Indian National Agricultural Research System
Content type Text
Educational Degree Doctor of Philosophy (Ph.D.)
Publisher CCSHAU
File Format PDF
Language English
Subject Domain (in DDC) Technology ♦ Agriculture & related technologies ♦ Techniques, equipment & materials
Subject Keyword Gene Effects ♦ Generation Mean Analysis ♦ Yellow Rust ♦ Ssr Markers ♦ Bread Wheat ♦ Genetics and Plant Breeding ♦ Molecular Characterization and Inheritance of Yellow Rust Resistance In Wheat (triticum Aestivum L. Em. Thell.)
Abstract The present investigation entitled “Molecular characterization and inheritance of yellow rust resistance in wheat (Triticum aestivum L. em. Thell.)†, was consisted of six generations i.e. parents (P1 and P2), F1, F2, BC1P1 and BC1P2 of eight crosses viz., DBW 17 x WH 1105, DBW 17 x RAJ 3765, DBW 17 x WH 1021, DBW 17 x WH 542, LASSIK x WH 542, LASSIK x WH 1021, LASSIK x RAJ 3765 and LASSIK x WH 1105 to study the inheritance and molecular aspects of yellow rust resistance and genetics of quantitative traits including grain yield and its component traits. The experiments were conducted in the Department of Genetics and Plant Breeding, CCS Haryana Agricultural University (CCSHAU), Hisar, India during two successive years of Rabi seasons 2014-15 and 2015-16. The field results have shown that inheritance of yellow rust was governed by two dominant genes which were also confirmed by SSR marker analysis. It has been observed that there was a strong association between field results for inheritance of yellow rust and SSR marker studied. Generation mean analysis revealed significant differences for all traits studied. The C and D scales were also significant for most of the characters indicating thereby the presence of non-allelic interactions. Additive component was significant for most of the characters and even as preponderant in magnitude over the dominance component. Additive x additive (i), additive x dominance (j) and dominant x dominant (l) type of interaction were reported significant for most of the characters. However, the magnitude and direction of the different gene effect estimates were changing from cross to cross. Additive component was found significant for most of the characters, however, dominance gene effects were also found significant for some characters. Duplicate type of interaction was recorded for the characters viz., spike weight and number of grains per spike in the cross DBW 17 x WH 1105, number of tillers per plant and number of grains per spike in the cross DBW 17 x RAJ 3765, number of tillers per plant, grain yield per plant and biological yield per plant in the cross DBW 17 x WH 1021, spike weight, number of spikelets per spike, grain yield per plant and biological yield pre plant in the cross DBW 17 x WH 542, spike weight in the cross LASSIK x WH 542, plant height and number of spikelets per spike in the cross LASSIK x WH 1021, plant height, spike length and number of grains per spike in the cross LASSIK x RAJ 3765 and spike length in the cross LASSIK x WH 1105. The differences between GCV and PCV values were less which showed least influence of environment. Grain yield per plant showed significant positive correlations with spike length, spike weight, 1000 grain weight, biological yield per plant. Total 136 SSR primers were used in the present study. Out of the 136 primers, 44 primers (including 12 Yr specific primers) were found to be polymorphic and 83 primers were monomorphic and 9 primers did not show amplification. It was confirmed by NTSYS-PC UPGMA cluster tree and dendrogram that two parents were most genetically diverse and the progeny lines were interspersed between the two parents for yellow rust in all the crosses.
Educational Use Research
Education Level UG and PG
Learning Resource Type Thesis
Size (in Bytes) 1.87 MB