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Researcher Vishalakshi
Advisor Gupta, Urmila
Source KrishiKosh-Indian National Agricultural Research System
Content type Text
Educational Degree Master of Science (M.Sc.)
Publisher Punjab Agricultural University
File Format PDF
Language English
Subject Domain (in DDC) Natural sciences & mathematics ♦ Life sciences; biology ♦ Microorganisms, fungi & algae
Subject Keyword Pleurotus Ostreatus ♦ Proximate and Chemical Analysis ♦ Paddy Straw Pretreatment ♦ Biogas Production ♦ Inoculum Concentration ♦ Lignocellulolytic Activity ♦ Development of Inoculum of Pleurotus Ostreatus Mtcc 142 On Low Cost Substrate For Paddy Straw Pretreatment and Biogas Production ♦ Microbiology ♦ Pleurotus Ostreatus Mtcc 142
Abstract The present study was aimed at the development of inoculum of Pleurotus ostreatus MTCC 142 on low-cost substrate for paddy straw pretreatment and biogas production. Paddy straw, predominately contains cellulose (32-47%), hemicellulose (19-27%), silica (6-12%) and lignin (5-24%) which are associated with each other through complex bonding. Therefore, pretreatment of paddy straw is required to use it as a potential substrate for biogas production. Different low-cost substrates like wheat straw, paddy straw, cow dung, wood shavings, wheat straw and charcoal combination, paddy straw and charcoal combination were used to prepare inoculum of P. ostreatus MTCC 142. Wheat straw mixed with charcoal (4:1) was found to be the most suitable substrate for inoculum production on the basis of mycelial impregnation, clump formation, moisture retention, contamination as well as proximate analysis, chemical analysis, and lignocellulolytic enzyme activities. The culture grown on selected substrate was used to pretreat chopped paddy straw. The maximum reduction in lignin content (52.26%) was observed on day 10 of incubation. Inoculum concentration and method of inoculum distribution were also optimized. Inoculum concentration of 20% with double layering showed maximum enhancement in paddy straw digestibility, lignocellulolytic activity, and biogas production. Lignocellulolytic enzyme production was also studied by inoculating biodigested slurry with the selected substrate. The inoculated biodigested slurry was used to produce biogas from 100 Kg of paddy straw. Thus, wheat straw and charcoal combination was found to be most suitable for preparation of master culture of P. ostreatus MTCC 142 which can be further propagated on biodigested slurry. The supplementation of enzyme activated biodigested slurry in paddy straw based digester at field scale produced 3.119m3 of biogas, whereas 0.256m3 biogas was observed to be produced in control.
Educational Use Research
Education Level UG and PG
Learning Resource Type Thesis
Publisher Place Ludhiana
Size (in Bytes) 677.69 kB
Page Count 69