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Researcher Kale, Sarika Kisan
Advisor Navale, A. M.
Source KrishiKosh-Indian National Agricultural Research System
Content type Text
Educational Degree Master of Science (M.Sc.)
Publisher MPKV
File Format PDF
Language English
Subject Domain (in DDC) Technology ♦ Agriculture & related technologies
Subject Keyword Shraddha ♦ Agricultural Microbiology
Abstract The present investigation was carried out with main object to see the effect of inoculation of endophytic bacterium Gluconacetobacter diazotrophicus on growth, nutrient uptake and yield of pearl millet (Pennisetum typhoides) cv. Shraddha. To achieve this, a field trial was conducted at Department of Plant Pathology and Agricultural Microbiology, M.P.K.V., Rahuri during summer season of 2012-13. The present investigation involved isolation, screening and characterization of Gluconacetobacter diazotrophicus isolated from pearl millet cv. Shraddha (RHRBH-8609 strain) as a laboratory experiment. The isolate showed peculiar characteristic growth on acidic LGIP semi solid medium. After incubation at 28 + 20C, it showed white pellicle initially, which was later turned into orange thick pellicle at the top of semi-solid medium in test tube. At the same time the medium turned colourless due to assimilation of bromothymol blue. The morphological studies showed that, the organism was rod shaped gram negative, with motility due to lateral or peritrichous flagella, non sporeforming, colonies were circular, smooth and yellowish orange in colour. The results of field experiment in general revealed that seed inoculation with Gluconacetobacter diazotrophicus (RHRBH- 8609 strain) showed significant increase in growth parameters viz., seed germination, seedling vigour index, number of leaves, height of plant, root length, dry matter weight of shoot and root, N and P uptake, grain yield and sugar quality parameters viz., brix per centage, reducing, non reducing and total sugar per centage. The grain yield obtained due to application with Gluconacetobacter diazotrophicus + 75 % recommended dose of N + PSB (37.33q/ha) was on par with application of 100 kg N/ha without Gluconacetobacter diazotrophicus and PSB inoculation (36.97q/ha). These findings explicitly indicated the possibility of saving nitrogenous fertilizer to an extent of 25 % N i.e. 27.17 kg Urea/ha, in pearl millet. The results of microbial population of Gluconacetobacter diazotrophicus revealed that Gluconacetobacter population in stem was decreased with increasing levels of fertilizer nitrogen application to pearl millet.
Educational Use Research
Education Level UG and PG
Learning Resource Type Thesis
Publisher Place Maharashtra
Size (in Bytes) 494.76 kB