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Researcher B, Manu
Advisor Bharadwaj, C.
Source KrishiKosh-Indian National Agricultural Research System
Content type Text
Educational Degree Master of Science (M.Sc.)
File Format PDF
Language English
Subject Domain (in DDC) Technology ♦ Agriculture & related technologies ♦ Techniques, equipment & materials
Subject Keyword €œmorpho-physiological and Molecular Characterization of Chickpea (cicer Arietinum L.) Genotypes For Terminal Drought Tolerance†♦ Genetics and Plant Breeding
Abstract Morphological, physiological and molecular characterization of thirty genotypes of chickpea were undertaken to investigate the morphological and physiological traits that contribute to drought tolerance and to unearth the genetic diversity in these genotypes. The experiment was laid out in a randomized block design with two replications each in two environments viz., normal and rainfed. The analysis of variance indicated the presence of sufficient variability among the genotypes. Correlations under stress conditions identified number of primary branches, number of secondary branches, biological yield per metre row, harvest Index, seedling root length and relative water content to be significantly correlated with seed yield per metre row (SYM). These traits can be used as selection indices for selecting genotypes with specific adaptation to drought stress conditions. Twenty two of the one hundred and twenty SSR primers tested reported polymorphism, amplifying 95 alleles generating 4.31 alleles per primer pair. Dendrogram based on Jaccard’s similarity coefficients grouped genotypes into four clusters based on genetic distances. There was a general consensus of grouping of genotypes based on their drought susceptibility index. Candidate genes for drought tolerance that had been validated in chickpea were deployed to identify the most promising genotypes. Only three out of these viz., SPS medi, CAD and EREB genes amplified. The association analysis of these genes with the physiological parameters indicated membrane stability index (MSI) in case of EREB and MSI and RWC in case of SPS medi to be associated with drought stress. The genotypes that emerged as most drought tolerant in the study in both physiological scoring based on RWC and MSI as well as on gene amplification with EREB and SPS medi were L550, Pusa Green 112 and ICC 92944. These can be used in chickpea breeding programme for development of genotypes with greater resilience to terminal drought stress
Educational Use Research
Education Level UG and PG
Learning Resource Type Thesis
Size (in Bytes) 4.06 MB