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Researcher Jahan, Tallat
Advisor Paital, Biswaranjan
Source KrishiKosh-Indian National Agricultural Research System
Content type Text
Educational Degree Master of Science (M.Sc.)
File Format PDF
Language English
Subject Domain (in DDC) Natural sciences & mathematics ♦ Animals (Zoology)
Subject Keyword Water Deprivation Stress ♦ Catalase Activity ♦ Mud Crab ♦ Scylla Serrata ♦ Zoology ♦ Water Deprivation Stress On Catalase Activity In Mud Crab
Abstract The mud crab Scylla serrata represents one of the most important estuarine edible crab. It is cultured in large numbers for feeding purpose due to its taste andh high nutritional values. After capture, these crabs are tied and kept in baskets and transported to different places. Hence they are exposed to dehydration condition for several days and water flow through their gill is disrupted. As a result, a concomitant increase in reactive oxygen species begins, trigerring oxidative stress. The aim of this study was to determine catalase activity in S. serrata under water deprivation stress in its different tissue. Then three groups of 5 crabs were exposed to three different conditions. One in natural habitat i.e. in sea water, second with open chelipeds in water deprivation condition and third with tied chelipeds in water deprivation condition. Activity of catalase and protein content were measured in gill, muscle and hepatopancreas sample. Almost all analysed tissues showed catalase activity that varied from one stress condition to another. The results also varied from one tissue to another. There was an increase in catalase activity in response to dehydration stress in gill and hepatopancreas while, there was an decrease in catalase activity in muscle was observed. It remained more high in set of crabs with tied legs that may indicate more oxidative stress. Protein content was tissue specific. Protein content showed an increase in muscle while there was decrease in protein content of gill and hepatopancreas was noticed. The decline pattern was sharp and prominent in hepatopancreas as compared to gills with respect to water deprived states. The zone of inhibition by using bacterial strain Streptococcus aureus indicates that gill had the higher activity followed by muscle and HP. The present study depicts that crabs with untied legs under sea water should be marketed and further analyses are required to confirm the compound(s) and that make(s) the zone of inhibition.
Educational Use Research
Education Level UG and PG
Learning Resource Type Thesis
Size (in Bytes) 1.68 MB
Page Count 49