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Researcher Sarika
Advisor Kumar, Rajinder
Source KrishiKosh-Indian National Agricultural Research System
Content type Text
Educational Degree Master of Science (M.Sc.)
Publisher LUVAS
File Format PDF
Language English
Subject Domain (in DDC) Technology ♦ Agriculture & related technologies ♦ Animal husbandry
Subject Keyword Anthelmintic Resistance ♦ Combination of Drugs ♦ Fenbendazole ♦ Haemonchus Contortus ♦ Ivermectin ♦ Levamisole ♦ Morantel ♦ Reversion ♦ Sheep ♦ Veterinary Parasitology ♦ Studies On The Status of Anthelmintic Resistance and Efficacy of Anthelmintic Combinations Against Gastrointestinal Nematodes In Sheep
Abstract This study was conducted to investigate the anthelmintic resistance status in nematodes infected sheep of the Sheep Breeding Farm, LLRUVAS, Hisar. Fifty sheep with faecal egg count per gram (epg) of ≥150 were divided into 5 groups. Groups I, II, III and IV were administered fenbendazole (5 mg/kg body weight orally), morantel(10 mg/kg body weight orally), levamisole(7.5 mg/kg body weight subcutaneously) and ivermectin (0.2 mg/kg body weight subcutaneously), respectively. Group V was the untreated control. Faecal samples were collected on 13th day after treatment from animals of all groups and individual faecal egg counts were determined by modified Mc Master technique. Faecal egg count reduction percentages in groups I, II, III and IV were 88.35, 97.26, 84.25 and 82.2%, respectively. Coproculture during the pretreatment period showed Haemonchus contortus as the predominant larvae (91%), followed by Strongyloides spp. (5%), Trichostrongylus spp. (2%), Oesophagostomum sp. (1%) and Bunostomum sp. (1%). After treatment with the anthelmintics, mainly the larvae of H. contortus were observed in the treated groups. In 1994-1995, faecal egg count reduction for fenbendazole, levamisole, ivermectin and morantel on the farm were 42, 24, 100 and 61%, respectively. This indicates partial reversion to susceptibility of fenbendazole and levamisole, resistance of ivermectin and complete reversion to susceptibility of morantel to resistant strain of H. contortus. Ivermectin resistance is recorded for the first time in sheep of this farm. The history revealed that after withdrawal of fenbendazole, morantel and levamisole, the farm has switched on to Ivermectin since 1995, though fenbandazole was used occasionally. A second experiment was conducted to investigate the efficacy of combinations of anthelmintics against gastrointestinal nematodes in sheep of the same farm. Forty eight sheep with faecal epg of ≥150 were divided into 4 groups. Groups I, II and III were administered fenbendazole + morantel, fenbendazole + levamisole and morantel+ levamisole, respectively. Dose of each drug was same as in first experiment. Group IV was the untreated control. Faecal egg count reduction percentages in groups I, II and III on 13th day post treatment were 97.96, 97.6 and 97.6%, respectively. Coproculture during the pretreatment period showed H. contortus as the predominant larvae (93%), followed by Strongyloides spp. (3%), Trichostrongylus spp. (2%), Oesophagostomum sp. (1%) and Bunostomum sp. (1%). After treatment with the anthelmintics, no larvae were observed in the treated groups. It can be concluded that the problem of anthelmintic resistance is spreading fast.This appears to be the first report on use of combinations of anthelmintics against resistant H.contortus in sheep from India.
Educational Use Research
Education Level UG and PG
Learning Resource Type Thesis
Size (in Bytes) 1.07 MB