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Researcher Agnihotri, Divya
Advisor Singh, Yudhvir
Source KrishiKosh-Indian National Agricultural Research System
Content type Text
Educational Degree Doctor of Philosophy (Ph.D.)
Publisher LUVAS
File Format PDF
Language English
Subject Domain (in DDC) Technology ♦ Agriculture & related technologies ♦ Animal husbandry
Subject Keyword Pcr ♦ Rt-pcr ♦ Canine Parvovirus ♦ Canine Coronavirus ♦ Canine Distemper Virus ♦ Gastroenteritis. ♦ Veterinary Medicine ♦ Molecular Characterization of Important Enteric Viruses and Their Therapeutic Management In Dogs
Abstract Important causative agents of canine viral enteritis include CPV, CDV and CCoV. An assay based on PCR and RT-PCR was designed to screen the diarrheal faecal samples of dogs for the presence of these pathogens followed their characterization by nucleotide sequencing. Out of 50 samples, 30 samples were found positive for these viral pathogens. Total percent prevalence of CPV, CCoV and CD were found to be 50%, 8% and 2% respectively. Sequencing of the CPV VP2 gene revealed all Hisar isolates to be of type CPV-2a showing maximum nucleotide identity with the Chinese isolates and with the commercial vaccine. N gene based partial sequencing of Hisar CDV isolate showed maximum identity with the Chinese isolate and low nucleotide identity with the commercial vaccine while Hisar CCoV isolate revealed maximum nucleotide identity with the Brazilian isolate and low nucleotide identity with the vaccine strain on the basis of partial M gene sequencing. Maximum occurrence of viral gastroenteritis was found in the unvaccinated mixed breed male dogs less than six months of age having black or tan coat colour. Anorexia, dehydration, lethargy, vomition and haemorrhagic diarrhea were the main clinical signs observed. Anaemia, leucopenia, neutropenia and thrombocytopenia with increased ALT, BUN and creatinine levels were observed in affected cases. Lower levels of total protein, albumin, globulin, sodium, potassium and chloride were observed in the diarrheic dogs. Treatment with Chloramphenicol and Ceftriaxone along with symptomatic and supportive treatment was found to be 90 per cent and 80 per cent effective respectively in the two treatment groups.
Educational Use Research
Education Level UG and PG
Learning Resource Type Thesis
Size (in Bytes) 3.30 MB