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Researcher Hirani, Nimishaben V.
Advisor Mehta, D. R.
Source KrishiKosh-Indian National Agricultural Research System
Content type Text
Educational Degree Master of Science (M.Sc.)
Publisher JAU
File Format PDF
Language English
Subject Domain (in DDC) Natural sciences & mathematics ♦ Life sciences; biology ♦ Microorganisms, fungi & algae ♦ Technology ♦ Agriculture & related technologies ♦ Techniques, equipment & materials
Subject Keyword Agricultural Biotechnology ♦ Biotechnology ♦ Diversity Analysis and Sex Determination In Spine Gourd (momordica Dioica Roxb.) Using Biochemical and Molecular Markers 2440
Abstract Spine gourd (Momordica dioica Roxb.) is the important in cucurbitaceae family vegetable crops with diploid chromosome number 2n = 28. The experiment was conducted at Biotechnology Laboratory, Department of Genetics and Plant Breeding, J.A.U., Junagadh during 2016-17. The experimental materials comprised of 10 genotypes of spine gourd (males and females) with two main objectives: (i) biochemical markers analysis through protein profiling using Native-PAGE and five isoenzymes viz., esterase, peroxidase, acid phosphatase and polyphenol oxidase (ii) molecular marker analysis through RAPD primers. Isoenzymes and protein profiling were used for the characterization of 10 spine gourd genotypes of male and female plants. The protein profile produced total of two monomorphic bands. Esterase and peroxidase isoenzymes each generated total of four bands. Out of four bands one was monomorphic and three were polymorphic in each case. Three bands of acid phosphatase isoenzyme were detected. Out of three, one was unique in GP-7 female genotype, one band was monomorphic and one band was polymorphic in nature. Two monomorphic bands were detected in polyphenol oxidase isoenzyme. Twenty-eight RAPD primers generated total of 182 bands/alleles in which 165 bands were polymorphic (90.66%) with. an average of 5.89 polymorphic bands per primer. Twenty-three primers demonstrated 100% polymorphism. The amplified fragments of RAPD varied from 110 bp (CMN-A01) to 2082 bp (CMN-A44). The polymorphic information content (PIC) was recorded from 0.3598 to 0.9011, while genetic similarity varied from 0.291 to 0.721. ii The pooled study of molecular marker through RAPD was done to confirm the differences and similarity between 10 spine gourd genotypes of male and female plants. The jaccard’s similarity coefficient and UPGMA method showed the highest (72.1%) similarity between GP-2 female and GP-4 female and the lowest (29.1%) similarity between GP-1 male and GP-8 female. The dendrogram consisted of two main clusters I and II with an average similarity of 41 %. The genotype identification through molecular and biochemical markers resulted in developing moderate diversified dendrogram of 10 spine gourd genotypes of male and female plants. The data revealed that molecular techniques are more accurate than biochemical markers, and can be used for genetic diversity analysis of spine gourd genotypes. Key words: Protein profiling, Isoenzymes, RAPD, Spine gourd
Educational Use Research
Education Level UG and PG
Learning Resource Type Thesis
Publisher Place Junagadh
Size (in Bytes) 237.27 kB