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Researcher Siddartha, H. S.
Advisor Narayanaswamy), P.
Source KrishiKosh-Indian National Agricultural Research System
Content type Text
Educational Degree Master of Science (M.Sc.)
Publisher University of Agricultural Sciences
File Format PDF
Language English
Subject Domain (in DDC) Natural sciences & mathematics ♦ Physics ♦ Light & infrared & ultraviolet phenomena
Subject Keyword Horticulture ♦ Estimation of Genetic Diversity In Jamun
Abstract Jamun is an important minor fruit of tropical and subtropical countries and widely distributed throughout India. It is a versatile tree for both food and medicinal values. Totally, sixty jamun individuals were analyzed in the present investigations. The leaf samples of genotypes were collected from GKVK i.e. in the Sericulture Department, Farm section and in and around Bangalore. About 32 accessions were collected from KRC college of Horticulture Arabhavi Belgaum. Randomly Amplified Polymorphic DNA (RAPD) markers have been used to estimate the genetic diversity among 60 genotypes of jamun. PCR amplifiable DNA was isolated using the CTAB method and 62 bands were amplified from 9 random 10-mer primers. The genetic dissimilarity matrix calculated based on Euclidean distance revealed a maximum genetic distance of 78 per cent between the genotype SERI 5 and SERI 2, while it was minimum (3 %) between Bangalore Local 1 and Bangalore Local 3. The present studies showed a moderate (3 %) to high (78 %) genetic diversity and in the dendrogram almost all the Arabhavi genotypes and FSO genotypes clustered together compared to others. This technique is useful in estimation of genetic diversity and also could be a first step towards efficient germplasm management of jamun in India.
Educational Use Research
Education Level UG and PG
Learning Resource Type Thesis
Publisher Place Bangalore
Size (in Bytes) 1.63 MB
Page Count 164