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Researcher Hinge, Pushpalata Nilakanth
Advisor Kale, A. A.
Source KrishiKosh-Indian National Agricultural Research System
Content type Text
Educational Degree Master of Science (M.Sc.)
Publisher Mahatma Phule Krishi Vidyapeeth
File Format PDF
Language English
Subject Domain (in DDC) Technology ♦ Agriculture & related technologies ♦ Techniques, equipment & materials
Subject Keyword Sorghum ♦ Agricultural Biotechnology
Abstract The present investigation was undertaken to study physiological and biochemical changes, molecular analysis of sorghum genotypes in response to PEG-6000 induced water stress. Six drought susceptible SPV 1359, SPV-1626, SPV-1830, SPV-504, SPV-1502 and RSV-1130, six drought tolerant SPV-1546, RSV-458, RSLG-262, SEL-3, M-35-1 and SPV 1704 and seven wild sorghum genotypes IS-18804, IS-18833, IS-18855, IS 18866, IS-18872, IS-18898, and IS-18909 were subjected to PEG-6000 induced water stress at -0.1,-0.2, -0.4, -0.6, -0.8 -1.0 and -1.2 MPa. Germination %, root length (cm), shoot length (cm), root to shoot ratio, chlorophyll content and chlorophyll stability index were decreased however membrane injury index was increased with increasing PEG-6000 induced water stress. It was noticed that drought tolerant genotypes had more chlorophyll a, total chlorophyll, chlorophyll stability index and less membrane injury index at each osmotic stress level. A drought tolerant genotype M-35-1 had maximum root length, shoot length, chlorophyll stability index and less membrane injury index than the rest of the genotypes. Based on the above result of physiological constituents, -0.1 MPa osmotic potential was selected for further biochemical analysis. It was revealed that the activity of P5CS increased due to water stress in all genotypes of sorghum which resulted in increased proline content in the seedlings. It was observed that in drought tolerant genotypes fold increase of proline was higher however; wild genotypes had higher absolute values under osmotic stress condition. The activity of P5CS was more pronounced in a drought tolerant genotype M-35-1 and wild genotype IS 18866 than rest of the genotypes. Soluble protein also increased under stressed condition in all sorghum genotypes in response to imposed water stress. Activities of antioxidant enzymes viz., SOD, POD, CAT increased under stressed condition in all sorghum genotypes. Activities of all these enzymes were higher in drought tolerant genotypes followed by those wild genotypes. Highest antioxidant enzyme activities were found in M 35-1 a drought tolerant genotype.
Educational Use Research
Education Level UG and PG
Learning Resource Type Thesis
Publisher Place Maharashtra
Size (in Bytes) 6.34 MB