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Researcher S, Nimmy M.
Advisor Srinivasan), R.
Source KrishiKosh-Indian National Agricultural Research System
Content type Text
Educational Degree Doctor of Philosophy (Ph.D.)
Publisher NATIONAL RESEARCH CENTRE ON PLANT BIOTECHNOLOGY INDIAN AGRICULTURAL RESEARCH INSTITUTE NEW DELHI
File Format PDF
Language English
Subject Domain (in DDC) Natural sciences & mathematics ♦ Life sciences; biology
Subject Keyword Isolation and Characterization of Salt Inducible Gene (s) / Promoter (s) From Chickpea ♦ Biotechnology and Molecular Biology
Abstract Salinity is one of the major abiotic stresses which adversely affect crop plants limiting growth and yield potential. In this study, by in silico analysis about 44 candidate genes in Arabidopsis that were previously annotated as genes involved in response to salt and were previously uncharacterized were identified on the basis of their putative function in salt tolerance. Gene expression profiling by semi quantitative and qRT PCR confirmed that six genes significantly upregulate under salt stress. A chickpea gene was identified which is an Arabidopsis ortholog, and the gene up regulates under salt stress specifically in roots compared with shoots which was detected by qRT PCR. Transcription start site of the gene was determined by 5’RACE. To further characterize the gene, over expressed in Arabidopsis and generated transgenic plants. Characterized the gene at molecular, physiological and biochemical level. Various studies such as germination assay, root length assay, salt tolerance assay, antioxidant enzyme activity assay carried out using T3 transgenic lines. ~2kb 5’ upstream promoter region was amplified and found to contain many abiotic stress responsive elements. Promoter fragment was used to transform Arabidopsis and to functionally validate the salt responsive promoter GUS assay was done in Arabidopsis transgenics.
Educational Use Research
Education Level UG and PG
Learning Resource Type Thesis
Size (in Bytes) 3.65 MB