Thumbnail
Access Restriction
Open

Researcher Verma, Anuradha
Advisor Swamy, Madhu
Source KrishiKosh-Indian National Agricultural Research System
Content type Text
Educational Degree Master of Science (M.Sc.)
Publisher NANAJI DESHMUKH VETERINARY SCIENCE UNIVERSITY JABALPUR
File Format PDF
Language English
Subject Domain (in DDC) Technology ♦ Agriculture & related technologies ♦ Animal husbandry
Subject Keyword Mannheimia Haemolytica ♦ Veterinary Pathology ♦ Applied Research
Abstract Pneumonia is one of the most common respiratory problems in small ruminants throughout the world. The most frequent causes of respiratory infection and death in goats are Pasteurella multocida or Mannheimia haemolytica (previously called Pasteurella haemolytica). The present study was designed to study the lobe distribution of lung pneumonic lesions in goats and to isolate and characterize P.multocida from cases of caprine pneumonia. Goats of different age groups and either sex bought for post-mortem examination at Department of Pathology or whose necropsy was conducted at Amanala Goat unit of College of Veterinary Science were included in the study. Total 97 post mortem examinations of goats were conducted and pneumonia was observed in 65 (67.03%) animals. Of these, the detailed study was conducted for fifty post mortem cases. In maximum cases (41/50, 82%), both the right and left lungs were affected. Areas of emphysema were observed in 100% cases, congestion in 82 %, consolidation in 66%, pulmonary edema in 46%, pleural adhesion in 08%, lung abscess in 04% and a solitary case (02%) had a nodular growth. On the basis of gross lung lesions maximum number of bronchopneumonia, 26/50; 52.0%, were diagnosed, followed by 23/50; 46.00% of interstitial pneumonia. Only a single case of granulomatous pneumonia was observed in the left cardiac lobe and no lung lesion fulfilling the criteria of embolic pneumonia was observed. The distribution of gross lesions in pneumonic lungs was further classified as multifocal, diffuse and local extensive. Maximum cases of pneumonia showed diffuse pattern of lesions and only ten cases were having a multifocal pattern of lung lesions. The lesion of solitary case of granulomatous pneumonia was classified as local extensive. Out of the 26 cases of bronchopneumonia 20 (76.92%) had features of suppurative bronchopneumonia and only 6 (23.07%) were of fibrinous bronchopneumonia. Tissue samples from most affected lobe of lung and lymph node were collected for histopathology. The distribution of microscopic lesions in the lung tissues comprising emphysema (84%), atelectasis (76%), congestion (70%), haemorrhage (46%), thickness of alveolar septa (84%), leukocytic infiltration (68%), fibrosis (76%) and calcification (04%) were observed. To determine the presence of Pasturella multocida in caprine pneumonia direct impression smears were prepared from all 50 cases. Typical bipolar organisms resembling Pasteurella spp. were observed in 32 cases. From the 50 cases of pneumonia selected, P. multocida was isolated and identified in 34 (68.0%) cases by Blood agar, Mac Conkey agar, Grams Staining and Biochemical tests. Nasal swabs were also taken aseptically from live goats at Amanala Goat unit which were showing symptoms of respiratory distress. Seven isolates of Pasturella multocida were obtained from the nasal swabs. Ten bacterial isolates obtained from pneumonic goats and nasal swabs having the biochemical characteristics of P. multocida were further subjected to in vitro antibiotic sensitivity test. The antibiogram pattern indicated that maximum percentage of Pasturella multocida were sensitive to Cotrioxazole (90%) followed by Gentamicin (80%) and Amoxyclav (80%). Pathogenicity of P.multocida bacillus isolated from pneumonic caprine lung was tested in albino rats. Pneumonic lungs were observed in 4/5 animals sacrificed at 48 hours and in 5/5 animals sacrificed at 72 hours. The gross lesions in affected lungs were of bronchopneumonia in all the cases. P. multocida was re-isolated from the lung tissue of rats at both the intervals.
Educational Use Research
Education Level UG and PG
Learning Resource Type Thesis
Size (in Bytes) 533.53 kB
Page Count 63