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Researcher Moharana, Baisakthi
Advisor Preetha, S.P. Selvasubramanian, S. Balachandran, C.
Source KrishiKosh-Indian National Agricultural Research System
Content type Text
Educational Degree Master of Science (M.Sc.)
Publisher TANUVAS
File Format PDF
Language English
Subject Domain (in DDC) Technology ♦ Agriculture & related technologies ♦ Animal husbandry
Subject Keyword Veterinary Science ♦ Veterinary Pharmacology and Toxicology ♦ Antitumour ♦ Nanoparticle ♦ Veterinary Science Veterinary Pharmacology and Toxicology
Abstract Nanomedicine deals with the use of precision-engineered nanomaterials to develop novel therapeutic and diagnostic modalities for human and animal use. Fractionated pectin powder (FPP) a well known antimetastatic agent and biologically synthesized silver nanoparticles (nAg particles) with potent antitumour properties were used in this study to formulate the nanocomposite i.e. nAg particle tagged FPP. The present study demonstrates the efficacy of nAg particle tagged FPP as an antitumor agent using Ehrlich ascites carcinoma (EAC) cell lines in vitro and in vivo. FPP, nAg particles and nAg particle tagged FPP at the two predetermined doses showed significant cytotoxicity against EAC cells. This inhibition was observed with reduced tumour volume, reduced viable cell count and with increased lifespan of the tumour induced animals. The mechanism that mediated tumour suppression was uncovered through depletion of mitochondrial potential suggesting activation of mitochondrial pathway, leading to induction of apoptosis which was further confirmed through phosphatidylserine externalization assay. Cell cycle analysis also revealed significant increase in the cells with sub-G1 DNA content. Further the antiangiogenic potential of various treatments were determined through significant reduction of peritoneal angiogenesis and VEGF levels in ascitic fluid. The antioxidant status, serum biochemical markers for cellular integrity and haematological parameters were restored to normalcy after various treatments. Histopathological analysis of the peritoneal lining showed attachment of increased number of degenerated and necrotic cells in the treated groups, indicating tumour cytotoxic potential of the various treatments. Real time quantitative analysis revealed a decrease in the expression of Bcl-2 and Gal-3 in the treated groups with increase in Bax expression in nAg particles-treated group. In this assessment, the effect of nAg particle tagged FPP at lower dose showed no significant difference from its higher dose counterpart and was found to be more effective in inducing apoptosis. By the present findings it is obvious that nAg particles tagged FPP was found to posess antitumour properties, which can be used as a cost-effective alternative in the treatment of cancer and angiogenesis-related disorders.
Educational Use Research
Education Level UG and PG
Learning Resource Type Thesis
Publisher Place Chennai
Size (in Bytes) 418.41 kB
Page Count 4