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Author Descostes, Nicolas ♦ Heidemann, Martin ♦ Spinelli, Lionel ♦ Schüller, Roland ♦ Maqbool, Muhammad Ahmad ♦ Fenouil, Romain ♦ Koch, Frederic ♦ Innocenti, Charlène ♦ Gut, Marta ♦ Gut, Ivo ♦ Eick, Dirk ♦ Andrau, Jean-Christophe
Source Paperity
Content type Text
Publisher eLife Sciences Publications, Ltd
File Format PDF ♦ HTM / HTML
Copyright Year ©2014
Abstract In mammals, the carboxy-terminal domain (CTD) of RNA polymerase (Pol) II consists of 52 conserved heptapeptide repeats containing the consensus sequence Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7. Post-translational modifications of the CTD coordinate the transcription cycle and various steps of mRNA maturation. Here we describe Tyr1 phosphorylation (Tyr1P) as a hallmark of promoter (5′ associated) Pol II in mammalian cells, in contrast to what was described in yeast. Tyr1P is predominantly found in antisense orientation at promoters but is also specifically enriched at active enhancers. Mutation of Tyr1 to phenylalanine (Y1F) prevents the formation of the hyper-phosphorylated Pol IIO form, induces degradation of Pol II to the truncated Pol IIB form, and results in a lethal phenotype. Our results suggest that Tyr1P has evolved specialized and essential functions in higher eukaryotes associated with antisense promoter and enhancer transcription, and Pol II stability.
Learning Resource Type Article
Publisher Date 2014-05-09
e-ISSN 2050084X
Journal eLife