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Author Ryoichi, Nakatsuji ♦ Hashida, Tomoko ♦ Matsumoto, Naoko ♦ Masato, Tsuro ♦ Kubo, Nakao ♦ Hirai, Masashi
Source PubMed Central
Content type Text
Publisher Japanese Society of Breeding
File Format PDF
Date Created 2011-12-15
Copyright Year ©2011
Language English
Difficulty Level Medium
Subject Domain (in DDC) Technology ♦ Medicine & health
Subject Keyword Agronomy and Crop Science ♦ Plant Science ♦ Genetics
Abstract Radish (Raphanus sativus L.) belongs to Brassicaceae family and is a close relative of Brassica. This species shows a wide morphological diversity, and is an important vegetable especially in Asia. However, molecular research of radish is behind compared to that of Brassica. For example, reports on SSR (simple sequence repeat) markers are limited. Here, we designed 417 radish SSR markers from SSR-enriched genomic libraries and the cDNA data. Of the 256 SSR markers succeeded in PCR, 130 showed clear polymorphisms between two radish lines; a rat-tail radish and a Japanese cultivar, ‘Harufuku’. As a test case for evaluation of the present SSRs, we conducted two studies. First, we selected 16 SSRs to calculate polymorphism information contents (PICs) using 16 radish cultivars and four other Brassicaceae species. These markers detected 3–15 alleles (average = 9.6). PIC values ranged from 0.54 to 0.92 (average = 0.78). Second, part of the present SSRs were tested for mapping using our previously-examined mapping population. The map spanned 672.7 cM with nine linkage groups (LGs). The 21 radish SSR markers were distributed throughout the LGs. The SSR markers developed here would be informative and useful for genetic analysis in radish and its related species.
ISSN 13447610
Age Range above 22 year
Educational Use Research
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2011-01-01
Rights Holder Japanese Society of Breeding
e-ISSN 13473735
Journal Breeding Science
Volume Number 61
Issue Number 4
Page Count 7
Starting Page 413
Ending Page 419

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Source: PubMed Central