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Author Zanelato, A. I. ♦ Shiota, V. M. ♦ Gomes, E. ♦ Silva, R. Da ♦ Thoméo, J. C.
Source PubMed Central
Content type Text
Publisher Sociedade Brasileira de Microbiologia
File Format PDF
Copyright Year ©2012
Language English
Difficulty Level Medium
Subject Domain (in DDC) Technology ♦ Medicine & health
Abstract This work is aimed to produce endoglucanase through solid state fermentation in a packed bed bioreactor with the use of the fungus Myceliophtora sp. I-1D3b using a mixture of wheat bran (WB) and sugar cane bagasse (SCB) as culture medium. Preliminary tests were performed in polypropylene plastic bags, controlling the variables temperature (40, 45, and 50°C), initial moisture content (75, 80, and 85%, w.b.), and weight proportion SCB/WB (1:1, 7:3, and 9:1). The highest enzyme activities in plastic bags were obtained using the substrate proportion of 7:3, 50°C temperature, and 80% initial moisture content (878 U/grams of dry solid). High activities of filter-paper cellulase and xylanase were also obtained in plastic bags and some results are reported. For the packed bed experiments, the temperature (45 and 50°C) and the air flow rate (80, 100 and 120L/h) were the controlled variables. Activity of endoglucanase was similar to plastic bag tests. A longitudinal gradient of moisture content, was observed increasing from the bottom to the top of the reactor, even though the longitudinal enzyme activity profile was flat for almost the whole bed. Air flow rate did not affect enzyme activity, while experiments carried out at 50°C showed higher enzyme activities. The maximum temperature peak observed was at about 6°C above the process temperature.
ISSN 16784405
Age Range above 22 year
Educational Use Research
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2012-01-01
Rights Holder Sociedade Brasileira de Microbiologia
e-ISSN 16784405
Journal Brazilian Journal of Microbiology
Volume Number 43
Issue Number 4
Starting Page 1536


Source: PubMed Central