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Author Wall, J. D. ♦ Murnan, T.
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword BIOLOGY AND MEDICINE, BASIC STUDIES ♦ DESULFOVIBRIO ♦ MUTAGENESIS ♦ GENES ♦ TRANSPOSONS ♦ EVALUATION
Abstract The transposons Tn5, Tn7, Tn9, and Tn10 or their derivatives have been examined for transposition in the sulfate-reducing bacterium Desulfovibrio desulfuricans G20. Tn7 inserted with a frequency of 10{sup {minus}4} to 10{sup {minus}3} into a unique attachment site that shows strong homology with those sites identified in other gram-negative bacteria. Inactivation of the tnsD gene in Tn7, encoding the function directing insertion into the unique site, yielded a derivative that transposed essentially randomly with a frequency of ca. 10{sup {minus}6} per donor. Derivatives of Tn5, but not wild-type Tn5, were also found to undergo random transposition at a similar frequency. No evidence was obtained for transposition of Tn9 or Tn10. 34 refs., 5 figs., 2 tabs.
ISSN 00992240
Educational Use Research
Learning Resource Type Article
Publisher Date 1996-10-01
Publisher Place United States
Journal Applied and Environmental Microbiology
Volume Number 62
Issue Number 10


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