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Author Liu, Lianyong ♦ Wang, Jingnan ♦ Li, Xiangqi ♦ Ma, Junhua ♦ Shi, Chao ♦ Zhu, Hongling ♦ Xi, Qian ♦ Zhang, Jichen ♦ Zhao, Xuemei ♦ Gu, Mingjun
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword APPLIED LIFE SCIENCES ♦ ANIMAL TISSUES ♦ APOPTOSIS ♦ CARCINOMAS ♦ CELL CYCLE ♦ CELL PROLIFERATION ♦ GROWTH FACTORS ♦ INSULIN ♦ LUCIFERASE ♦ MESSENGER-RNA ♦ PLANT GROWTH ♦ THYROID
Abstract microRNAs (miRNAs) are frequently dysregulated in human malignancies. It was recently shown that miR-204-5p is downregulated in papillary thyroid carcinoma (PTC); however, the functional significance of this observation is not known. This study investigated the role of miR-204-5p in PTC. Overexpressing miR-204-5p suppressed PTC cell proliferation and induced cell cycle arrest and apoptosis. The results of a luciferase reporter assay showed that miR-204-5p can directly bind to the 3′ untranslated region (UTR) of insulin-like growth factor-binding protein 5 (IGFBP5) mRNA, and IGFBP5 overexpression partially reversed the growth-inhibitory effects of miR-204-5p. These results indicate that miR-204-5p acts as a tumor suppressor in PTC by regulating IGFBP5 expression and that miR-204-5p can potentially serve as an antitumorigenic agent in the treatment of PTC. - Highlights: • miR-204-5p expression is downregulated in PTC tissues and cell lines. • miR-204-5p suppresses proliferation and promotes apoptosis in PTC cells. • miR-204-5p suppresses IGFBP5 expression by direct binding to the 3′-UTR. • IGFBP5 overexpression reverses the effects of miR-204-5p.
ISSN 0006291X
Educational Use Research
Learning Resource Type Article
Publisher Date 2015-02-20
Publisher Place United States
Journal Biochemical and Biophysical Research Communications
Volume Number 457
Issue Number 4


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