|Author||Zhu, Wenzhen ♦ Yang, Bingwu ♦ Fu, Huiling ♦ Ma, Long ♦ Liu, Tingting ♦ Chai, Rongfei ♦ Zheng, Zhaodi ♦ Zhang, Qunye ♦ Li, Guorong|
|Source||United States Department of Energy Office of Scientific and Technical Information|
|Subject Keyword||APPLIED LIFE SCIENCES ♦ APOPTOSIS ♦ BIOTIN ♦ CELL PROLIFERATION ♦ FLAVONOIDS ♦ FLUORESCENCE ♦ MAMMARY GLANDS ♦ MICROSCOPY ♦ MODIFICATIONS ♦ NEOPLASMS ♦ NITRIC OXIDE ♦ PLANT GROWTH ♦ PROTEINS ♦ SYNTHESIS|
|Abstract||As the core structure of flavonoids, flavone has been proved to possess anticancer effects. Flavone's growth inhibitory functions are related to NO. NO is synthesized by nitric oxide synthase (NOS), and generally increased in a variety of cancer cells. NO regulates multiple cellular responses by S-nitrosylation. In this study, we explored flavone-induced regulations on nitric oxide (NO)-related cellular processes in breast cancer cells. Our results showed that, flavone suppresses breast cancer cell proliferation and induces apoptosis. Flavone restrains NO synthesis by does-dependent inhibiting NOS enzymatic activity. The decrease of NO generation was detected by fluorescence microscopy and flow cytometry. Flavone-induced inhibitory effect on NOS activity is dependent on intact cell structure. For the NO-induced protein modification, flavone treatment significantly down-regulated protein S-nitrosylation, which was detected by “Biotin-switch” method. The present study provides a novel, NO-related mechanism for the anticancer function of flavone. - Highlights: • Flavone inhibits proliferation and induces apoptosis in MCF-7 cells. • Flavone decreases nitric oxide production by inhibiting NOS enzymatic activity in breast cancer cells. • Flavone down-regulates protein S-nitrosylation.|
|Learning Resource Type||Article|
|Publisher Place||United States|
|Journal||Biochemical and Biophysical Research Communications|
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