|Author||DeMarini, D. M. ♦ Cros, S. ♦ Paoletti, C. ♦ Lecointe, P. ♦ Hsie, A. W.|
|Source||United States Department of Energy Office of Scientific and Technical Information|
|Subject Keyword||RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT. ♦ ANTINEOPLASTIC DRUGS ♦ CARCINOGEN SCREENING ♦ MUTAGEN SCREENING ♦ ANIMAL CELLS ♦ CELL KILLING ♦ CHEMICAL BONDS ♦ CHO CELLS ♦ COVALENCE ♦ DNA REPLICATION ♦ ENZYME ACTIVITY ♦ EXPERIMENTAL DATA ♦ HAMSTERS ♦ METABOLIC ACTIVATION ♦ MUTAGENESIS ♦ REVERTANTS ♦ SALMONELLA ♦ STRUCTURE-ACTIVITY RELATIONSHIPS ♦ TOXICITY ♦ ANIMALS ♦ BACTERIA ♦ DATA ♦ DRUGS ♦ INFORMATION ♦ MAMMALS ♦ MICROORGANISMS ♦ MUTANTS ♦ NUCLEIC ACID REPLICATION ♦ NUMERICAL DATA ♦ RODENTS ♦ SCREENING ♦ VERTEBRATES ♦ Chemicals Metabolism & Toxicology- Cells- (-1987) ♦ Chemicals Metabolism & Toxicology- Microorganisms- (-1987)|
|Abstract||The mutagenicity and cytotoxicity of five antitumor compounds (ellipticines) were investigated in the Chinese hamster ovary cell hypoxanthine-guanine phosphoribosyltransferase assay and in six strains of Salmonella. All five compounds (ellipticine, 9-methoxyellipticine, 9-hydroxyellipticine, 9-aminoellipticine, and 2-methyl-9-hydroxyellipticinium) were cytotoxic and mutagenic in the Chinese hamster ovary cell hypoxanthine-guanine phosphoribosyltransferase assay in the presence or absence of Aroclor 1254-induced rat liver S9, and all except the last compound were mutagenic in Salmonella. Based on the reversion pattern obtained in various frame-shift and DNA repair-proficient (uvrB/sup +/) or -deficient (uvrB) strains of Salmonella in the presence or absence of S9, the first three compounds appear to cause frameshift mutations by both intercalation and covalent bonding with DNA; thus, these are classified as reactive intercalators. However, 9-aminoellipticine intercalates only weakly and may instead exert its mutagenic activity primarily (or exclusively) by forming a covalent adduct with DNA. Compared to the published antitumor data obtained in mice, the results in Salmonella and Chinese hamster ovary cells suggest that the ability of ellipticine, 9-methoxyellipticine, and 9-hydroxyellipticine to intercalate with DNA, induce frame-shift mutations, and cause cell killing is associated with and may be the basis for their antitumor activity. The observation that the ellipticines are mutagenic in mammalian cells suggests that these antitumor agents may be carcinogenic.|
|Learning Resource Type||Article|
|Publisher Place||United States|
|Organization||Univ. of Tennessee-Oak Ridge Graduate School of Biomedical Sciences|
National Digital Library of India (NDLI) is a virtual repository of learning resources which is not just a repository with search/browse facilities but provides a host of services for the learner community. It is sponsored and mentored by Ministry of Education, Government of India, through its National Mission on Education through Information and Communication Technology (NMEICT). Filtered and federated searching is employed to facilitate focused searching so that learners can find the right resource with least effort and in minimum time. NDLI provides user group-specific services such as Examination Preparatory for School and College students and job aspirants. Services for Researchers and general learners are also provided. NDLI is designed to hold content of any language and provides interface support for 10 most widely used Indian languages. It is built to provide support for all academic levels including researchers and life-long learners, all disciplines, all popular forms of access devices and differently-abled learners. It is designed to enable people to learn and prepare from best practices from all over the world and to facilitate researchers to perform inter-linked exploration from multiple sources. It is developed, operated and maintained from Indian Institute of Technology Kharagpur.
NDLI is a conglomeration of freely available or institutionally contributed or donated or publisher managed contents. Almost all these contents are hosted and accessed from respective sources. The responsibility for authenticity, relevance, completeness, accuracy, reliability and suitability of these contents rests with the respective organization and NDLI has no responsibility or liability for these. Every effort is made to keep the NDLI portal up and running smoothly unless there are some unavoidable technical issues.
Ministry of Education, through its National Mission on Education through Information and Communication Technology (NMEICT), has sponsored and funded the National Digital Library of India (NDLI) project.
For any issue or feedback, please write to firstname.lastname@example.org