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Author Ohe, Shuichi ♦ Tanaka, Toshihiro ♦ Yanai, Hirotsugu ♦ Komai, Yoshihiro ♦ Omachi, Taichi ♦ Kanno, Shohei ♦ Tanaka, Kiyomichi ♦ Ishigaki, Kazuhiko ♦ Saiga, Kazuho ♦ Nakamura, Naohiro ♦ Ohsugi, Haruyuki ♦ Tokuyama, Yoko ♦ Atsumi, Naho ♦ Hisha, Hiroko ♦ Yoshida, Naoko ♦ Kumano, Keiki ♦ Yamazaki, Fumikazu ♦ Okamoto, Hiroyuki ♦ Ueno, Hiroo
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword APPLIED LIFE SCIENCES ♦ CELL CYCLE ♦ EPIDERMIS ♦ GLANDS ♦ GTP-ASES ♦ HEALING ♦ IRRADIATION ♦ LEUCINE ♦ LYMPHOMAS ♦ MICE ♦ RECEPTORS ♦ STEM CELLS ♦ SWEAT ♦ WOUNDS
Abstract The skin is responsible for a variety of physiological functions and is critical for wound healing and repair. Therefore, the regenerative capacity of the skin is important. However, stem cells responsible for maintaining the acral epithelium had not previously been identified. In this study, we identified the specific stem cells in the acral epithelium that participate in the long-term maintenance of sweat glands, ducts, and interadnexal epidermis and that facilitate the regeneration of these structures following injury. Lgr6-positive cells and Bmi1-positive cells were found to function as long-term multipotent stem cells that maintained the entire eccrine unit and the interadnexal epidermis. However, while Lgr6-positive cells were rapidly cycled and constantly supplied differentiated cells, Bmi1-positive cells were slow to cycle and occasionally entered the cell cycle under physiological conditions. Upon irradiation-induced injury, Bmi1-positive cells rapidly proliferated and regenerated injured epithelial tissue. Therefore, Bmi1-positive stem cells served as reservoir stem cells. Lgr5-positive cells were rapidly cycled and maintained only sweat glands; therefore, we concluded that these cells functioned as lineage-restricted progenitors. Taken together, our data demonstrated the identification of stem cells that maintained the entire acral epithelium and supported the different roles of three cellular classes. - Highlights: • The acral epithelium have two types of stem cells. • Lgr6-positive cells are rapid-cycling, short-term stem cells. • Bmi1-positive cells are slow-cycling stem cells that act as reserver stem cells. • Lgr5 may be a useful sweat gland marker in mice.
ISSN 0006291X
Educational Use Research
Learning Resource Type Article
Publisher Date 2015-10-23
Publisher Place United States
Journal Biochemical and Biophysical Research Communications
Volume Number 466
Issue Number 3


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