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Author Filipović, Selma ♦ Aljičević, Mufida ♦ Smajilagić, Amer ♦ Redžić, Amira ♦ Lagumdžija, Alena C.
Source Directory of Open Access Journals (DOAJ)
Content type Text
Publisher Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina
File Format HTM / HTML
Date Created 2017-11-09
Copyright Year ©2013
Language English
Subject Domain (in LCC) R5-920
Subject Keyword Bone marrow stem cells ♦ Medicine ♦ Bone marrow ♦ Adult stem cells
Abstract Bone marrow mesenchymal cells have been identified as a source of pluripotent stem cells with multipotential potential and differentiation in to the different cells types such as are osteoblast, chondroblast, adipoblast. In this research we describe pioneering experiment of tissue engineering in Bosnia and Herzegovina, of the isolation and differentiation rat bone marrow stromal cells in to the osteoblast cells lineages. Rat bone marrow stromal cells were isolated by method described by Maniatopulos using their plastic adherence capatibility. The cells obtained by plastic adherence were cultured and serially passaged in the osteoinductive medium to differentiate into the osteocytes. Bone marrow samples from rats long bones used for isolation of stromal cells (BMSCs). Under determinate culture conditions BMSCs were differentiated in osteogenic cell lines detected by Alizarin red staining three weeks after isolation. BMSCs as autologue cells model showed high osteogenetic potential and calcification capatibility in vitro. In future should be used as alternative method for bone transplantation in Regenerative Medicine.
ISSN 15128601
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study
Learning Resource Type Article
Publisher Date 2013-02-01
e-ISSN 15128601
Journal Bosnian Journal of Basic Medical Sciences
Volume Number 13
Issue Number 1
Page Count 4
Starting Page 27
Ending Page 30


Source: Directory of Open Access Journals (DOAJ)