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Author Morozov, A. N. ♦ Turchin, Il'ya V. ♦ Kamenskii, V. A. ♦ Fiks, I. I. ♦ Lazutkin, A. A. ♦ Bezryadkov, D. V. ♦ Ivanova, A. A. ♦ Toptunov, D. M. ♦ Anokhin, K. V.
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword RADIOLOGY AND NUCLEAR MEDICINE ♦ BRAIN ♦ EMBRYOS ♦ EXCITATION ♦ FIBER OPTICS ♦ FLUORESCENCE ♦ HEART ♦ IMAGES ♦ LASER RADIATION ♦ LUNGS ♦ MICE ♦ MICROSCOPY ♦ OPACITY ♦ SPATIAL RESOLUTION ♦ THREE-DIMENSIONAL CALCULATIONS ♦ ANIMALS ♦ BODY ♦ CARDIOVASCULAR SYSTEM ♦ CENTRAL NERVOUS SYSTEM ♦ ELECTROMAGNETIC RADIATION ♦ EMISSION ♦ ENERGY-LEVEL TRANSITIONS ♦ LUMINESCENCE ♦ MAMMALS ♦ NERVOUS SYSTEM ♦ OPTICAL PROPERTIES ♦ OPTICS ♦ ORGANS ♦ PHOTON EMISSION ♦ PHYSICAL PROPERTIES ♦ RADIATIONS ♦ RESOLUTION ♦ RESPIRATORY SYSTEM ♦ RODENTS ♦ VERTEBRATES
Abstract The method of fluorescent microscopy is developed based on employment of a single-mode fibreoptic channel to provide high spatial resolution 3D images of large cleared biological specimens using the 488-nm excitation laser line. The transverse and axial resolution of the setup is 5 and 13 {mu}m, respectively. The transversal sample size under investigation is up to 10 mm. The in-depth scanning range depends on the sample transparency and reaches 4 mm in the experiment. The 3D images of whole mouse organs (heart, lungs, brain) and mouse embryos obtained using autofluorescence or fluorescence of exogenous markers demonstrate a high contrast and cellular-level resolution.
ISSN 10637818
Educational Use Research
Learning Resource Type Article
Publisher Date 2010-11-13
Publisher Place United States
Journal Quantum Electronics
Volume Number 40
Issue Number 9


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