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Author Wu, Cheng-Jang ♦ Lu, Chun-Hao ♦ Chen, Li-Chen ♦ Nguyen, Duc T. ♦ Huang, Yi-Shu ♦ Lin, Hsi-Hsien ♦ Lin, Chun-Yen ♦ Kuo, Ming-Ling
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword APPLIED LIFE SCIENCES ♦ ANTIGENS ♦ ASTHMA ♦ CALCIUM ♦ CELL PROLIFERATION ♦ GENE REGULATION ♦ IN VITRO ♦ IN VIVO ♦ INFLAMMATION ♦ INHIBITION ♦ LIPIDS ♦ MICE ♦ MONOCLONAL ANTIBODIES ♦ OVA
Abstract Non-depleting YTS177 anti-CD4 monoclonal antibody (MoAb) has been reported to lead to antigen-specific immunotolerance in allograft transplantation and autoimmune diabetes, as well as possibly to inhibition of allergic inflammation in mice. However, the molecular mechanisms underlying hyporesponsive T cell responses induced by YTS177 MoAb remain elusive. Herein, we demonstrate that the YTS177 MoAb increases the levels of anergy factors p27{sup kip1} and Cbl-b, inhibits IL-2 production, and impairs calcium mobilization in activated T cells in vitro. YTS177 MoAb suppresses OVA-driven proliferation of DO11.10 CD4{sup +} T cells in vivo as well. Mechanistically, YTS177 MoAb induces tolerance by causing CD4 down-regulation through clathrin-dependent and raft dissociation. The results obtained in this study lead us to propose novel protective or curative approaches to CD4 T cell-mediated diseases.
ISSN 0006291X
Educational Use Research
Learning Resource Type Article
Publisher Date 2016-05-13
Publisher Place United States
Journal Biochemical and Biophysical Research Communications
Volume Number 473
Issue Number 4


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