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Author Ropars, C. ♦ Chassaigne, M. ♦ Avenard, G.
Source SpringerLink
Content type Text
Publisher Kluwer Academic Publishers
File Format PDF
Copyright Year ©1998
Language English
Subject Domain (in DDC) Technology ♦ Engineering & allied operations
Subject Keyword Inositol-hexaphosphate ♦ P$_{50}$ ♦ Oxygen transport ♦ Oxemia ♦ Hypoxia ♦ Ischemia ♦ Oxyhaemoglobin dissociation curve ♦ Rightward shift ♦ Human Physiology ♦ Neurosciences ♦ Imaging ♦ Radiology ♦ Computer Applications ♦ Biomedical Engineering
Abstract The red blood cell (RBC) membrane may be reversibly opened using a lysis-resealing continuous flow method. The technology was adapted to the internalisation of an allosteric effector of haemoglobin, Inostiol-Hexaphosphate (IHP). This molecule, occupying the allosteric site of 2,3 Bis-Phosphoglycerate with a very large affinity, induces a rightward shift of the oxyhaemoglobin dissociation curve (ODC). From ODC parameters in human volunteers, the potential effect of P$_{50}$ (oxygen pressure at 50% haemoglobin saturation) on oxygen exchangeable fraction (OEF%), for various oxygen partial pressures (oxemia) was evaluated. For hyperoxic or normoxic arterial oxygen pressure (paO$_{2}$), rightward shift greatly improved OEF%. In optimised conditions, engineered erythrocytes were potentially able to deliver two to three times more oxygen than normal cells. For patients with decreased paO$_{2}$, as observed in chronic obstructive pulmonary deficiency (COPD), the reduction in arterial oxygen saturation (saO$_{2}$%) reduces the benefit of the treatment for paO$_{2}$ values between 60 and 80 mmHg. Below 60 mmHg, the saO$_{2}$% reduction cannot be compensated by a corresponding reduction in svO$_{2}$%, particularly for organs with physiologically low svO$_{2}$%. In these organs, deleterious effects could be observed for a very large rightward shift of the ODC. Such engineered cells have unique properties for oxygen transport improvement and may be used for the treatment of patients suffering from diseases associated with hypoxia and ischemia.
ISSN 01400118
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG
Learning Resource Type Article
Publisher Date 1998-01-01
Publisher Place Dordrecht
e-ISSN 17410444
Journal Medical and Biological Engineering and Computing
Volume Number 36
Issue Number 4
Page Count 5
Starting Page 508
Ending Page 512

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Source: SpringerLink