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Author Betala, Pravin A. ♦ Appugounder, Suganya ♦ Chakraborty, Sudipto ♦ Songprawat, Preedarat ♦ Buttner, William J. ♦ Perez Luna, Victor H.
Source SpringerLink
Content type Text
Publisher Springer-Verlag
File Format PDF
Copyright Year ©2007
Language English
Subject Domain (in DDC) Natural sciences & mathematics ♦ Chemistry & allied sciences
Subject Keyword Colorimetric detection ♦ Escherichia coli ♦ Antibodies ♦ Antigens ♦ Gold nanoparticles ♦ Silver reduction ♦ Engineering ♦ Chemistry/Food Science ♦ Food Science
Abstract A colorimetric immunoassay has been developed based on the reduction and precipitation of silver ions catalyzed by colloidal gold. This method was evaluated for a noncompetitive heterogeneous immunoassay to detect ovalbumin as a model protein and Escherichia coli as model bacterium. The influence of relevant experimental variables, including the reaction time of antigen with antibody, the dilution ratio of the colloidal gold-labeled protein, time allowed for silver reduction/precipitation reaction and other parameters were examined and optimized. This colorimetric method of detection is convenient, easy to use and economic. The presence of the target molecules or antigens can be visually inferred based on the color change that occurs in a short time without using major instrumentation. This method is widely amenable for detection of proteins, viruses and bacteria.
ISSN 19327587
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2007-12-05
Publisher Place New York
e-ISSN 19329954
Journal Sensing and Instrumentation for Food Quality and Safety
Volume Number 2
Issue Number 1
Page Count 9
Starting Page 34
Ending Page 42

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Source: SpringerLink