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Author Yang, Jian ♦ Jiang, Hongchen ♦ Wu, Geng ♦ Hou, Weiguo ♦ Sun, Yongjuan ♦ Lai, Zhongping ♦ Dong, Hailiang
Source SpringerLink
Content type Text
Publisher SP Higher Education Press
File Format PDF
Copyright Year ©2012
Language English
Subject Domain (in DDC) Natural sciences & mathematics ♦ Earth sciences
Subject Keyword anammox ♦ n-damo ♦ co-occurrence ♦ saline lakes ♦ Qinghai-Tibetan Plateau ♦ Earth Sciences
Abstract Nitrite-dependent anaerobic methane-oxidizing (n-damo) bacteria and anaerobic ammonia oxidizing (anammox) bacteria are two groups of microorganisms involved in global carbon and nitrogen cycling. In order to test whether the n-damo and anammox bacteria co-occur in natural saline environments, the DNA and cDNA samples obtained from the surficial sediments of two saline lakes (with salinity of 32 and 84 g/L, respectively) on the Tibetan Plateau were PCR-amplified with the use of anammox- and n-damo-specific primer sets, followed by clone library construction and phylogenetic analysis. DNA and cDNA-based clones affiliated with n-damo and anammox bacteria were successfully retrieved from the two samples, indicating that these two groups of bacteria can co-occur in natural saline environments with salinity as high as 84 g/L. Our finding has great implications for our understanding of the global carbon and nitrogen cycle in nature.
ISSN 20950195
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2012-09-20
Publisher Institution Chinese Universities
Publisher Place Heidelberg
e-ISSN 20950209
Journal Frontiers of Earth Science in China
Volume Number 6
Issue Number 4
Page Count 9
Starting Page 383
Ending Page 391

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Source: SpringerLink