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Author Ma, Yue ping ♦ Wei, Jiang xue ♦ Yu, Zhi yang ♦ Qin, Bing ♦ Dai, Si lan
Source SpringerLink
Content type Text
Publisher Northeast Forestry University
File Format PDF
Copyright Year ©2015
Language English
Subject Domain (in DDC) Natural sciences & mathematics ♦ Life sciences; biology
Subject Keyword Chrysanthemum L. ♦ Flow cytometry ♦ OTTO extraction buffer ♦ Ploidy identification ♦ Forestry
Abstract Analyzing the ploidy levels of plants is important for identifying species, selecting parental lines, identifying the relationships between species, and determining evolutionary patterns. The genus Chrysanthemum is widely distributed throughout the world and exhibits different ploidy levels. We used flow cytometry to analyze the ploidy levels of nine species of Chrysanthemum L. collected from different regions and geographical locations in China. Three diploids from Henan and Wuhan provinces corresponded to Chrysanthemum lavandulifolium and two species of C. nankingense, while three tetraploids from various regions corresponded to C. indicum and two species of C. chanetii. Two hexaploids corresponding to C. vestitum were collected at Funiu mountain (Henan province), and C. zawadskii was collected at Huangshan mountain (Anhui province). We found that OTTO extraction buffer was suitable for extracting nuclei from most species, apart from C. zawadskii. Flow cytometry proved to be a simple, rapid, and highly accurate method for identifying ploidy levels in Chrysanthemum species.
ISSN 1007662X
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2015-04-30
Publisher Place Harbin
e-ISSN 19930607
Journal Journal of Forestry Research
Volume Number 26
Issue Number 3
Page Count 5
Starting Page 771
Ending Page 775

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Source: SpringerLink