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Author DEWEY, D. L.
Sponsorship USDOE
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Publisher Nature Publishing Group
Language English
Subject Keyword RADIOLOGY AND NUCLEAR MEDICINE ♦ ALCOHOLS ♦ BACTERIA ♦ FREE RADICALS ♦ GLYCERIN ♦ OXYGEN ♦ RADIATION PROTECTION ♦ RADIOSENSITIVITY ♦ SERRATIA ♦ VARIATIONS
Abstract Radiation protection by glycerine was examined using the smaIl red- pignnented bacterium Serratia marcescens. Observations indicated that the protection of aerobic organisms was brought about by some mechanism other than oxygen scavenging or competition with oxygen for radioinduced radicals. Organisms that were anaerobic when irradiated were also substantially protected by the presence of glycerine. Overnight cultures grown in unaerated nutrient were diluted in phosphate buffer and suspended in glycerine solution a few minutes before irradiation. The protective effect of glycerine was found to be independent of a change in oxygen concentration by a factor of 100 and also independent of temperature changes. The ratio of sensitivity of the bacteria in the absence of glycerine to its sensitivity in glycerine was found to be 5.25.
ISSN 00280836
Educational Use Research
Learning Resource Type Article
Publisher Date 1960-09-17
Publisher Department Mount Vernon Hospital, Northwood, Middx., Eng.
Journal Nature
Volume Number 187
Issue Number 4742
Organization Mount Vernon Hospital, Northwood, Middx., Eng.