Thumbnail
Access Restriction
Open

Author Jang, Byeong-Churl
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword APPLIED LIFE SCIENCES ♦ ADENOSINE ♦ CARBOXYLIC ACIDS ♦ CATTLE ♦ CHEMILUMINESCENCE ♦ CONCENTRATION RATIO ♦ LACTONES ♦ LEPTIN ♦ MESSENGER-RNA ♦ METABOLIC DISEASES ♦ PHENOTYPE ♦ PHOSPHORYLATION ♦ RECEPTORS ♦ TRANSCRIPTION ♦ TRIGLYCERIDES
Abstract Differentiation of preadipocyte, also called adipogenesis, leads to the phenotype of mature adipocyte. However, excessive adipogenesis is closely linked to the development of obesity. Artesunate, one of artemisinin-type sesquiterpene lactones from Artemisia annua L., is known for anti-malarial and anti-cancerous activities. In this study, we investigated the effect of artesunate on adipogenesis in 3T3-L1 preadipocytes. Artesunate strongly inhibited lipid accumulation and triglyceride (TG) synthesis during the differentiation of 3T3-L1 preadipocytes into adipocytes at 5 μM concentration. Artesunate at 5 μM also reduced not only the expressions of CCAAT/enhancer-binding protein-α (C/EBP-α), peroxisome proliferator-activated receptor-γ (PPAR-γ), fatty acid synthase (FAS), and perilipin A but also the phosphorylation levels of signal transducer and activator of transcription-3 (STAT-3) during adipocyte differentiation. Moreover, artesunate at 5 μM reduced leptin, but not adiponectin, mRNA expression during adipocyte differentiation. Taken together, these findings demonstrate that artesunate inhibits adipogenesis in 3T3-L1 preadipoytes through the reduced expression and/or phosphorylation levels of C/EBP-α, PPAR-γ, FAS, perilipin A, and STAT-3. -- Highlights: •Artesunate, an artemisinin derivative, inhibits adipogenesis. •Artesunate inhibits C/EBP-α, PPAR-γ, FAS, perilipin A, and STAT-3 in 3T3-L1 adipocytes. •Artesunate reduces leptin, but not adiponectin, expression in 3T3-L1 adipocytes. •Artesunate thus may have therapeutic potential against obesity.
ISSN 0006291X
Educational Use Research
Learning Resource Type Article
Publisher Date 2016-05-20
Publisher Place United States
Journal Biochemical and Biophysical Research Communications
Volume Number 474
Issue Number 1


Open content in new tab

   Open content in new tab