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Author Tarnowka, M. A.
Source CiteSeerX
Content type Text
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Subject Domain (in DDC) Computer science, information & general works ♦ Data processing & computer science
Subject Keyword Tetvahymena Pyriformis St ♦ Heat-synchronized Cell Cycle ♦ Three Rnaases ♦ Short Communication Change ♦ Tetrahymenapyriformis St ♦ Synchronous Growth ♦ Stepwise Increase ♦ Total Rnaase Activity ♦ Major Peak ♦ Sulphopropyl Sephadex Chromatography ♦ Preferential Increase ♦ First Hour ♦ Major Rnaases ♦ Polyacrylamide Gel Electrophoresis ♦ Acid Rnaases ♦ Heat-synchronized Cell ♦ Heat Shock
Abstract Acid RNAases of Tetrahymenapyriformis ST were resolved into one minor and two major peaks by Sulphopropyl Sephadex chromatography. The same three peaks were obtained from exponentially growing cells and from heat-synchronized cells. The two major RNAases (peaks 2 and 3) were also separated by polyacrylamide gel electrophoresis at pH 4.5 and 8.3. The stepwise increase in total RNAase activity during synchronous growth was primarily caused by a large and preferential increase in the activity of the peak 3 RNAase during the first hour after the heat shock.
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study
Publisher Date 1978-01-01